Anti-FENS1/WDFY1 Antibody Picoband®, FITC Conjugate

• Background:

  The protein encoded by this gene is a phosphatidylinositol 3-phosphate binding protein, which contains a FYVE zinc finger domain and multiple WD-40 repeat domains. When exogenously expressed, it localizes to early endosomes. Mutagenesis analysis demonstrates that this endosomal localization is mediated by the FYVE domain.

• Description:

  Boster Bio Anti-FENS1/WDFY1 Antibody Picoband® catalog # A10318-3. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Pre T-cell antigen receptor alpha; pT-alpha; pTa; pT-alpha-TCR; PTCRA

• Gene Name:

  pre T cell antigen receptor alpha

• Gene ID:

  57590

• UniProt:

  Q8IWB7

• Host:

  Rabbit

• Reactivity:

  Human,Mouse,Rat

• Cross Reactivity:

  No cross-reactivity with other proteins.

• Immunogen:

  E.coli-derived human FENS1/WDFY1 recombinant protein (Position: H65-D394).

• Clonality:

  Polyclonal

• Tissue Specificity:

  Expressed in immature but not mature T-cells. Also found in CD34+ cells from peripheral blood, CD34+ precursors from umbilical cord blood and adult bone marrow.

• Applications:

  WB,Flow Cytometry,ELISA

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Function:

  The pre-T-cell receptor complex (composed of PTCRA, TCRB and the CD3 complex) regulates early T-cell development. .

• Components:

  Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

• References & Citations:

  1. Dutta, S., Roy, S., Polavaram, N. S., Baretton, G. B., Muders, M. H., Batra, S., Datta, K. NRP2 transcriptionally regulates its downstream effector WDFY1. Sci. Rep. 6: 23588, 2016. Note: Electronic Article. 2. Gross, M. B. Personal Communication. Baltimore, Md. 8/8/2018. 3. Hu, Y.-H., Zhang, Y., Jiang, L.-Q., Wang, S., Lei, C.-Q., Sun, M.-S., Shu, H.-B., Liu, Y. WDFY1 mediates TLR3/4 signaling by recruiting TRIF. EMBO Rep. 16: 447-455, 2015.

• Storage Conditions:

  At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

• Calculated Molecular Weight:

  41409 MW

• Observed Molecular Weight:

  46 kDa

• Fragment:

  Rabbit IgG

• Applications Notes:

  Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
  Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
  ELISA, 0.1-0.5 μg/ml, -
  

• Subcellular Location:

  Membrane; Single-pass type I membrane protein.

• Protein Name:

  Protein NDRG3