Anti-GP210/NUP210 Antibody Picoband®, FITC Conjugate

• Background:

  The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a membrane-spanning glycoprotein that is a major component of the nuclear pore complex. Multiple pseudogenes related to this gene are located on chromosome 3.

• Description:

  Boster Bio Anti-GP210/NUP210 Antibody Picoband® catalog # A05308. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Loricrin; LOR; LRN

• Gene Name:

  loricrin

• Gene ID:

  23225

• UniProt:

  Q8TEM1

• Host:

  Rabbit

• Reactivity:

  Human,Rat

• Cross Reactivity:

  No cross-reactivity with other proteins.

• Immunogen:

  E.coli-derived human GP210/NUP210 recombinant protein (Position: R221-K1724).

• Clonality:

  Polyclonal

• Tissue Specificity:

  Expressed in testis and to a lesser degree in brain, ovary and placenta. Found in most tissues at low levels.

• Applications:

  ELISA,Flow Cytometry,IF,IHC,ICC,WB

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Function:

  Major keratinocyte cell envelope protein.

• Components:

  Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

• References & Citations:

  1. Favreau, C., Worman, H. J., Wozniak, R. W., Frappier, T., Courvalin, J.-C. Cell cycle-dependent phosphorylation of nucleoporins and nuclear pore membrane protein Gp210. Biochemistry 35: 8035-8044, 1996. 2. Nagase, T., Ishikawa, K., Suyama, M., Kikuno, R., Hirosawa, M., Miyajima, N., Tanaka, A., Kotani, H., Nomura, N., Ohara, O. Prediction of the coding sequences of unidentified human genes. XII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. DNA Res. 5: 355-364, 1998. 3. Olsson, M., Ekblom, M., Fecker, L., Kurkinen, M., Ekblom, P. cDNA cloning and embryonic expression of mouse nuclear pore membrane glycoprotein 210 mRNA. Kidney Int. 56: 827-838, 1999.

• Storage Conditions:

  At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

• Calculated Molecular Weight:

  39411 MW

• Observed Molecular Weight:

  250 kDa

• Fragment:

  Rabbit IgG

• Applications Notes:

  Western blot, 0.1-0.25 μg/ml, Human, Rat
  Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
  Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
  Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
  ELISA, 0.1-0.5 μg/ml, -
  

• Subcellular Location:

  Nucleoplasm. Cytoplasm.

• Protein Name:

  Zinc finger protein Helios