Anti-EEF2K Antibody Picoband®, FITC Conjugate

• Background:

  Eukaryotic elongation factor-2 kinase (eEF-2 kinase or eEF-2K), also known as calmodulin-dependent protein kinase III (CAMKIII) and calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase, is an enzyme that in humans is encoded by the EEF2K gene. This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment.

• Description:

  Boster Bio Anti-EEF2K Antibody Picoband® catalog # A02277-3. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Arachidonate 12-lipoxygenase, 12S-type;12S-LOX;12S-lipoxygenase;1.13.11.31;Lipoxin synthase 12-LO;3.3.2.-;Platelet-type lipoxygenase 12;ALOX12;12LO, LOG12;

• Gene Name:

  Arachidonate 12-lipoxygenase, 12S-type

• Gene ID:

  29904

• UniProt:

  O00418

• Host:

  Rabbit

• Reactivity:

  Human,Rat

• Cross Reactivity:

  No cross-reactivity with other proteins.

• Immunogen:

  E.coli-derived human EEF2K recombinant protein (Position: K162-A719).

• Clonality:

  Polyclonal

• Tissue Specificity:

  Expressed in vascular smooth muscle cells. .

• Applications:

  ELISA,Flow Cytometry,IF,ICC,WB

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Function:

  Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Mainly converts arachidonic acid to (12S)- hydroperoxyeicosatetraenoic acid/ (12S)-HPETE but can also metabolize linoleic acid. Has a dual activity since it also converts leukotriene A4/LTA4 into both the bioactive lipoxin A4/LXA4 and lipoxin B4/LXB4. Through the production of specific bioactive lipids like (12S)-HPETE it regulates different biological processes including platelet activation. It also probably positively regulates angiogenesis through regulation of the expression of the vascular endothelial growth factor. Plays a role in apoptotic process, promoting the survival of vascular smooth muscle cells for instance. May also play a role in the control of cell migration and proliferation. .

• Components:

  Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

• References & Citations:

  1. Arora, S., Yang, J.-M., Craft, J., Hait, W. Detection of anti-elongation factor 2 kinase (calmodulin-dependent protein kinase III) antibodies in patients with systemic lupus erythematosus. Biochem. Biophys. Res. Commun. 293: 1073-1076, 2002. 2. Autry, A. E., Adachi, M., Nosyreva, E., Na, E. S., Los, M. F., Cheng, P., Kavalali, E. T., Monteggia, L. M. NMDA receptor blockade at rest triggers rapid behavioural antidepressant responses. Nature 475: 91-95, 2011. 3. Ryazanov, A. G., Ward, M. D., Mendola, C. E., Pavur, K. S., Dorovkov, M. V., Wiedmann, M., Erdjument-Bromage, H., Tempst, P., Parmer, T. G., Prostko, C. R., Germino, F. J., Hait, W. N. Identification of a new class of protein kinases represented by eukaryotic elongation factor-2 kinase. Proc. Nat. Acad. Sci. 94: 4884-4889, 1997.

• Storage Conditions:

  At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

• Calculated Molecular Weight:

  75694 MW

• Observed Molecular Weight:

  82 kDa

• Fragment:

  Rabbit IgG

• Applications Notes:

  Western blot, 0.25-0.5 μg/ml, Human, Rat
  Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
  Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
  ELISA, 0.1-0.5 μg/ml, -
  

• Subcellular Location:

  Cytoplasm, cytosol. Membrane. Membrane association is stimulated by EGF.

• Protein Name:

  Arachidonate 12-lipoxygenase, 12S-type