Anti-cPLA2 beta/PLA2G4B Antibody Picoband®, FITC Conjugate

• Background:

  Phospholipase A2, group IVB (cytosolic), also known as PLA2G4B, is a human gene. This gene encodes a member of the cytosolic phospholipase A2 protein family. Phospholipase A2 enzymes hydrolyze the sn-2 bond of phospholipids, releasing lysophospholipids and fatty acids. This enzyme may be associated with mitochondria and early endosomes. Most tissues also express read-through transcripts from the upstream gene into this gene, some of which may encode fusion proteins combining the N-terminus of the upstream gene including its JmjC domain with the almost complete coding region of this gene, including the C2 and cytoplasmic phospholipase A2 domains.

• Description:

  Boster Bio Anti-cPLA2 beta/PLA2G4B Antibody Picoband® catalog # A11137-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Interleukin-17B; IL-17B; Cytokine CX1; Cytokine-like protein ZCYTO7; Neuronal interleukin-17-related factor; Il17b; Nirf, Zcyto7

• Gene Name:

  interleukin 17B

• Gene ID:

  100137049

• UniProt:

  P0C869

• Host:

  Rabbit

• Reactivity:

  Human,Mouse,Rat

• Cross Reactivity:

  No cross-reactivity with other proteins.

• Immunogen:

  E.coli-derived human cPLA2 beta/PLA2G4B recombinant protein (Position: N61-H781).

• Clonality:

  Polyclonal

• Tissue Specificity:

  Expressed in adult pancreas, small intestine, stomach, spinal cord and testis. Less pronounced expression in prostate, colon mucosal lining, and ovary.

• Applications:

  ELISA,Flow Cytometry,WB

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

• Function:

  Stimulates the release of tumor necrosis factor alpha and IL-1-beta from the monocytic cell line THP-1. .

• Components:

  Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

• References & Citations:

  1. Pickard, R. T., Strifler, B. A., Kramer, R. M., Sharp, J. D. Molecular cloning of two new human paralogs of 85-kDa cytosolic phospholipase A2. J. Biol. Chem. 274: 8823-8831, 1999.

• Storage Conditions:

  Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

• Observed Molecular Weight:

  88 kDa

• Fragment:

  Rabbit IgG

• Applications Notes:

  Western blot, 0.25-0.5μg/ml, Mouse, Rat
  Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
  ELISA, 0.1-0.5μg/ml, -
  

• Subcellular Location:

  Secreted.