Anti-Superoxide Dismutase 1/SOD1 Antibody Picoband®, HRP Conjugate

• Background:

  Superoxide dismutases (SOD) are a class of enzymes that catalyze the dismutation of superoxide into oxygen and hydrogen peroxide. As such, they are an important antioxidant defense in nearly all cells exposed to oxygen. One of the exceedingly rare exceptions is Lactobacillus plantarum and related lactobacilli, which use a different mechanism.Cu,Zn-SOD was found widely distributed in the cell cytosol and in the cell nucleus, consistent with it being a soluble cytosolic protein. Mitochondria and secretory compartments did not label for this protein. In human cells, peroxisomes showed a labeling density slightly less than that of cytoplasm.

• Description:

  Boster Bio Anti-Superoxide Dismutase 1/SOD1 Antibody catalog # PA1345. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Superoxide dismutase [Cu-Zn];1.15.1.1;Superoxide dismutase 1;hSod1;SOD1;

• Gene Name:

  Superoxide dismutase [Cu-Zn]

• UniProt:

  P00441

• Host:

  Rabbit

• Reactivity:

  Human,Mouse,Rat

• Cross Reactivity:

  No cross-reactivity with other proteins

• Immunogen:

  A synthetic peptide corresponding to a sequence at the C-terminus of Human SOD1, different from the mouse sequence by two amino acids.

• Clonality:

  Polyclonal

• Tissue Specificity:

  Expressed in neurons of the rolandic area of the brain (at protein level). Expressed in the brain.

• Applications:

  IHC,ICC,WB

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

• Function:

  Destroys radicals which are normally produced within the cells and which are toxic to biological systems.

• Components:

  Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

• References & Citations:

  1. Crapo, J. D., Oury, T., Rabouille, C., Slot, J. W., Chang, L.-Y. Copper,zinc superoxide dismutase is primarily a cytosolic protein in human cells. Proc. Nat. Acad. Sci. 89: 10405-10409, 1992.

• Storage Conditions:

  Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  15936 MW

• Observed Molecular Weight:

  18 kDa

• Fragment:

  Rabbit IgG

• Specificity:

  No cross reactivity with other proteins.

• Applications Notes:

  Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
  Immunocytochemistry , 0.5-1μg/ml, Human, Mouse, Rat
  Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Mouse, Rat, Human
  Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
  

• Subcellular Location:

  Cytoplasm. Nucleus. Predominantly cytoplasmic; the pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria.

• Sequence Similarities:

  Belongs to the Cu-Zn superoxide dismutase family.

• Protein Name:

  Superoxide dismutase[Cu-Zn]