Anti-MTHFD1 Antibody Picoband®, HRP Conjugate

• Background:

  Monofunctional C1-tetrahydrofolate synthase, mitochondrial also known as formyltetrahydrofolate synthetase, is an enzyme that in humans is encoded by the MTHFD1L gene (methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like). The protein encoded by this gene is involved in the synthesis of tetrahydrofolate (THF) in the mitochondrion. THF is important in the de novo synthesis of purines and thymidylate and in the regeneration of methionine from homocysteine. Several transcript variants encoding different isoforms have been found for this gene.

• Description:

  Boster Bio Anti-MTHFD1 Antibody Picoband® catalog # A02025-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Tripartite motif-containing protein 6; RING finger protein 89; RING-type E3 ubiquitin transferase TRIM6; TRIM6; RNF89

• Gene Name:

  tripartite motif containing 6

• Gene ID:

  4522

• UniProt:

  P11586

• Host:

  Rabbit

• Reactivity:

  Human,Mouse,Rat

• Cross Reactivity:

  No cross-reactivity with other proteins.

• Immunogen:

  E.coli-derived human MTHFD1 recombinant protein (Position: K21-Q855).

• Clonality:

  Polyclonal

• Tissue Specificity:

  Isoform 2 is only expressed in skeletal muscle. Isoform 1 is expressed in skeletal muscle, heart, and in lesser extent in liver or pancreas. .

• Applications:

  WB,IHC,ICC,IF,Flow Cytometry,ELISA

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Function:

  E3 ubiquitin-protein ligase which ubiquitinates MYC and inhibits its transcription activation activity, maintaining the pluripotency of embryonic stem cells. Involved in the synthesis of unanchored K48-linked polyubiquitin chains which interact with and activate the serine/threonine kinase IKBKE, leading to phosphorylation of STAT1 and stimulation of an antiviral response .

• Components:

  Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

• References & Citations:

  1. Christensen, K. E., Patel, H., Kuzmanov, U., Mejia, N. R., MacKenzie, R. E. Disruption of the Mthfd1 gene reveals a monofunctional 10-formyltetrahydrofolate synthetase in mammalian mitochondria. J. Biol. Chem. 280: 7597-7602, 2005. 2. Momb, J., Lewandowski, J. P., Bryant, J. D., Fitch, R., Surman, D. R., Vokes, S. A., Appling, D. R. Deletion of Mthfd1l causes embryonic lethality and neural tube and craniofacial defects in mice. Proc. Nat. Acad. Sci. 110: 549-554, 2013. 3. Prasannan, P., Pike, S., Peng, K., Shane, B., Appling, D. R. Human mitochondrial C(1)-tetrahydrofolate synthase: gene structure, tissue distribution of the mRNA, and immunolocalization in Chinese hamster ovary cells. J. Biol. Chem. 278: 43178-43187, 2003.

• Storage Conditions:

  At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

• Calculated Molecular Weight:

  37492 MW

• Observed Molecular Weight:

  102 kDa

• Fragment:

  Rabbit IgG

• Applications Notes:

  Western blot, 0.1-0.25 μg/ml/ml, Human, Mouse, Rat
  Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml/ml, Human, Rat
  Immunocytochemistry/Immunofluorescence, 5 μg/ml/ml, Human
  Immunofluorescence, 5 μg/ml/ml, Human
  Flow Cytometry (Fixed), 1-3 μg/ml/1x10^6 cells, Human
  ELISA, 0.1-0.5 μg/ml/ml, Human
  

• Subcellular Location:

  Cytoplasm.

• Protein Name:

  Tripartite motif-containing protein 6