Anti-TMS1/ASC/Pycard Antibody Picoband®, HRP Conjugate

• Background:

  PYCARD, often referred to as ASC (Apoptosis-associated speck-like protein containing a CARD), is a protein that in humans is encoded by the PYCARD gene. This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene.

• Description:

  Boster Bio Anti-TMS1/ASC/Pycard Antibody Picoband® catalog # A00362-4. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Transcription factor ETV6; ETS translocation variant 6; ETS-related protein Tel1; Tel; ETV6; TEL; TEL1

• Gene Name:

  ETS variant 6

• Gene ID:

  66824

• UniProt:

  Q9EPB4

• Host:

  Rabbit

• Reactivity:

  Mouse,Rat

• Cross Reactivity:

  No cross-reactivity with other proteins.

• Immunogen:

  E.coli-derived mouse TMS1/ASC/PYCARD recombinant protein (Position: R3-S193).

• Clonality:

  Polyclonal

• Tissue Specificity:

  Ubiquitous.

• Applications:

  WB,IHC,Flow Cytometry,ELISA

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

• Function:

  Transcriptional repressor; binds to the DNA sequence 5'- CCGGAAGT-3'. Plays a role in hematopoiesis and malignant transformation.

• Components:

  Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.

• References & Citations:

  1. Agostini, L., Martinon, F., Burns, K., McDermott, M. F., Hawkins, P. N., Tschopp, J. NALP3 forms an IL-1-beta-processing inflammasome with increased activity in Muckle-Wells autoinflammatory disorder. Immunity 20: 319-325, 2004. 2. Cai, X., Chen, J., Xu, H., Liu, S., Jiang, Q.-X., Halfmann, R., Chen, Z. J. Prion-like polymerization underlies signal transduction in antiviral immune defense and inflammasome activation. Cell 156: 1207-1222, 2014. 3. Conway, K. E., McConnell, B. B., Bowring, C. E., Donald, C. D., Warren, S. T., Vertino, P. M. TMS1, a novel proapoptotic caspase recruitment domain protein, is a target of methylation-induced gene silencing in human breast cancers. Cancer Res. 60: 6236-6242, 2000.

• Storage Conditions:

  Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

• Observed Molecular Weight:

  22-25 kDa

• Fragment:

  Rabbit IgG

• Applications Notes:

  Western blot, 0.25-0.5μg/ml, Mouse, Rat
  Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse
  Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Mouse
  ELISA, 0.1-0.5μg/ml, -
  

• Subcellular Location:

  Nucleus.