Anti-MEK5/MAP2K5 Antibody Picoband®, HRP Conjugate

• Background:

  Dual specificity mitogen-activated protein kinase kinase 5 is an enzyme that in humans is encoded by the MAP2K5 gene. The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been described.

• Description:

  Boster Bio Anti-MEK5/MAP2K5 Antibody Picoband® catalog # A03980-3. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Intercellular adhesion molecule 2; ICAM-2; CD102; ICAM2

• Gene Name:

  intercellular adhesion molecule 2

• Gene ID:

  5607

• UniProt:

  Q13163

• Host:

  Rabbit

• Reactivity:

  Human,Monkey,Rat

• Cross Reactivity:

  No cross-reactivity with other proteins.

• Immunogen:

  E.coli-derived human MEK5/MAP2K5 recombinant protein (Position: F9-R442).

• Clonality:

  Polyclonal

• Tissue Specificity:

  Highly expressed in lung. Also detected in pancreas, kidney, small intestine, ovary, testis, prostate and mammary gland. In lung, it is found in alveolar type II cells but not in bronchiolar epithelium.

• Applications:

  ELISA,Flow Cytometry,IF,IHC,ICC,WB

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

• Function:

  ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen- specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance.

• Components:

  Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

• References & Citations:

  1. Zhou, G., Bao, Z. Q., Dixon, J. E. Components of a new human protein kinase signal transduction pathway. J. Biol. Chem. 270: 12665-12669, 1995.

• Storage Conditions:

  Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

• Observed Molecular Weight:

  50 kDa

• Fragment:

  Rabbit IgG

• Applications Notes:

  Western blot, 0.25-0.5μg/ml, Human, Monkey, Rat
  Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
  Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
  Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
  ELISA, 0.1-0.5μg/ml, -
  

• Subcellular Location:

  Membrane; Single-pass type I membrane protein.

• Protein Name:

  Eukaryotic initiation factor 4A-I