Anti-EEF2 Antibody Picoband®, HRP Conjugate

• Background:

  Eukaryotic elongation factor 2 is a protein that in humans is encoded by the EEF2 gene. This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation.

• Description:

  Boster Bio Anti-EEF2 Antibody Picoband® catalog # A00830-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat, Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Protein argonaute-1;Argonaute1;hAgo1;Argonaute RISC catalytic component 1;Eukaryotic translation initiation factor 2C 1;eIF-2C 1;eIF2C 1;Putative RNA-binding protein Q99;AGO1;EIF2C1;

• Gene Name:

  Protein argonaute-1

• Gene ID:

  1938

• UniProt:

  P13639

• Host:

  Rabbit

• Reactivity:

  Human,Monkey,Mouse,Rat,Zebrafish

• Cross Reactivity:

  No cross-reactivity with other proteins

• Immunogen:

  E.coli-derived human EEF2 recombinant protein (Position: M1-L858).

• Clonality:

  Polyclonal

• Tissue Specificity:

  Detected in blood plasma (at protein level).

• Applications:

  ELISA,IHC,WB

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

• Function:

  Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs). .

• Components:

  Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

• References & Citations:

  1. Davydova, E., Ho, A. Y. Y., Malecki, J., Moen, A., Enserink, J. M., Jakobsson, M. E., Loenarz, C., Falnes, P. O. Identification and characterization of a novel evolutionarily conserved lysine-specific methyltransferase targeting eukaryotic translation elongation factor 2 (eEF2). J. Biol. Chem. 289: 30499-30510, 2014. 2. Hekman, K. E., Yu, G.-Y., Brown, C. D., Zhu, H., Du, X., Gervin, K., Undlien, D. E., Peterson, A., Stevanin, G., Clark, H. B., Pulst, S. M., Bird, T. D., White, K. P., Gomez, C. M. A conserved eEF2 coding variant in SCA26 leads to loss of translational fidelity and increased susceptibility to proteostatic insult. Hum. Molec. Genet. 21: 5472-5483, 2012. 3. Kaneda, Y., Hayes, H., Uchida, T., Yoshida, M. C., Okada, Y. Regional assignment of five genes on human chromosome 19. Chromosoma 95: 8-12, 1987.

• Storage Conditions:

  Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  97214 MW

• Observed Molecular Weight:

  95 kDa

• Fragment:

  Rabbit IgG

• Applications Notes:

  Western blot, 0.1-0.25μg/ml, Human, Monkey, Mouse, Rat, Zebrafish
  Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat
  ELISA, 0.1-0.5μg/ml, -
  

• Subcellular Location:

  Cytoplasm, P-body .

• Protein Name:

  Protein argonaute-1