Anti-PPM1D/WIP1 Picoband® Antibody, HRP Conjugate

• Background:

  Protein phosphatase 1D is an enzyme that in humans is encoded by the PPM1D gene. It is mapped to 17q23.2. The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. The expression of this gene is induced in a p53-dependent manner in response to various environmental stresses. While being induced by tumor suppressor protein TP53/p53, this phosphatase negatively regulates the activity of p38 MAP kinase, MAPK/p38, through which it reduces the phosphorylation of p53, and in turn suppresses p53-mediated transcription and apoptosis. This phosphatase thus mediates a feedback regulation of p38-p53 signaling that contributes to growth inhibition and the suppression of stress induced apoptosis. This gene is located in a chromosomal region known to be amplified in breast cancer. The amplification of this gene has been detected in both breast cancer cell line and primary breast tumors, which suggests a role of this gene in cancer development.

• Description:

  Boster Bio Anti-PPM1D/WIP1 Picoband® Antibody catalog # A01576-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Protein phosphatase 1D; Protein phosphatase 2C isoform delta; PP2C-delta; Protein phosphatase magnesium-dependent 1 delta; p53-induced protein phosphatase 1; PPM1D; WIP1

• Gene Name:

  protein phosphatase, Mg2+/Mn2+ dependent 1D

• Gene ID:

  8493

• UniProt:

  O15297

• Host:

  Rabbit

• Reactivity:

  Human,Mouse,Rat

• Cross Reactivity:

  No cross-reactivity with other proteins.

• Immunogen:

  E.coli-derived human PPM1D/WIP1 recombinant protein (Position: R18-C605).

• Clonality:

  Polyclonal

• Tissue Specificity:

  Plasma.

• Applications:

  WB,ICC,IF,Flow Cytometry,ELISA

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

• Function:

  Involved in the negative regulation of p53 expression. Required for the relief of p53-dependent checkpoint mediated cell cycle arrest. Binds to and dephosphorylates 'Ser-15' of TP53 and 'Ser-345' of CHEK1 which contributes to the functional inactivation of these proteins. Mediates MAPK14 dephosphorylation and inactivation. Is also an important regulator of global heterochromatin silencing and critical in maintaining genome integrity.

• Components:

  Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

• References & Citations:

  1. Jansen, S., Geuer, S., Pfundt, R., Brough, R., Ghongane, P., Herkert, J. C., Marco, E. J., Willemsen, M. H., Kleefstra, T., Hannibal, M., Shieh, J. T., Lynch, S. A., and 18 others. De novo truncating mutations in the last and penultimate exons of PPM1D cause an intellectual disability syndrome. Am. J. Hum. Genet. 100: 650-658, 2017. 2. Li, J., Yang, Y., Peng, Y., Austin, R. J., van Eyndhoven, W. G., Nguyen, K. C. Q., Gabriele, T., McCurrach, M. E., Marks, J. R., Hoey, T., Lowe, S. W., Powers, S. Oncogenic properties of PPM1D located within a breast cancer amplification epicenter at 17q23. Nature Genet. 31: 133-134, 2002. 3. Lu, X., Nannenga, B., Donehower, L. A. PPM1D dephosphorylates Chk1 and p53 and abrogates cell cycle checkpoints. Genes Dev. 19: 1162-1174, 2005.

• Storage Conditions:

  Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  28681 MW

• Observed Molecular Weight:

  75 kDa

• Fragment:

  Rabbit IgG

• Specificity:

  No cross reactivity with other proteins.

• Applications Notes:

  Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
  Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
  Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
  ELISA, 0.1-0.5μg/ml, -
  

• Subcellular Location:

  Nucleus. Cytosol.

• Protein Name:

  40S ribosomal protein S6