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Human IgM Fab mu

CAT: 0013-GTR19063765Size: 2 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR19063765Size:2 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Human IgM Fab mu
Product Name Alternative
Human Myeloma Fragment, fab fragment of a human
Conjugation
Unconjugated
Source
Human
Origin
Human
Field of Research
Epigenetics & Chromatin
Concentration
2.0 mg/mL
Dilution
ELISA: User Optimized, WB: User Optimized
Purity
HUMAN IgM (myeloma) Fab μ fragment was prepared from serum by a multi-step process which includes delipidation, selective precipitation, tandem molecular sieve chromatography and trypsin digestion followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Serum. No reaction was observed against anti-Human IgG F (c) . Some light chain cross reactivity will occur with anti-Human IgG.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Monovalent Fab fragments of affinity-purified, secondary antibodies are offered to cover (block) the surface of immunoglobulins for double labeling primary antibodies from the same host species, or to block endogenous immunoglobulins in tissue sections or on cell surfaces. They can be used for these purposes because each Fab fragment has only a single antigen binding site. HUMAN IgM (myeloma) Fab μ fragment has been tested in SDS-PAge and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments. Specific conditions should be optimized by the user
Tested Applications
SDS-PAGE
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

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