Anti-EAAT2/GLT-1/SLC1A2 Antibody Picoband® (HRP Conjugate)

• Background:

  SLC1A2 is also known as EAAT2 or GLT-1. This gene encodes a member of a family of solute transporter proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors. Mutations in and decreased expression of this protein are associated with amyotrophic lateral sclerosis. Alternatively spliced transcript variants of this gene have been identified.

• Description:

  Boster Bio Anti-EAAT2/GLT-1/SLC1A2 Antibody catalog # RP1065. Tested in IF, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Excitatory amino acid transporter 2;Glutamate/aspartate transporter II;Sodium-dependent glutamate/aspartate transporter 2;Solute carrier family 1 member 2;SLC1A2;EAAT2, GLT1;

• Gene Name:

  Excitatory amino acid transporter 2

• UniProt:

  P43004

• Host:

  Rabbit

• Reactivity:

  Mouse,Rat

• Cross Reactivity:

  No cross-reactivity with other proteins

• Immunogen:

  E.coli-derived human EAAT2 recombinant protein (Position: T461-K574). Human EAAT2 shares 96% amino acid (aa) sequence identity with both mouse and rat EAAT2.

• Clonality:

  Polyclonal

• Applications:

  IF,IHC,WB

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

• Function:

  Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.

• Storage Conditions:

  Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

• Fragment:

  Rabbit IgG

• Applications Notes:

  Western blot, 0.1-0.5μg/ml, Mouse, Rat
  Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat
  Immunofluorescence, 5 μg/ml, Mouse, Rat
  

• Subcellular Location:

  Membrane; Multi-pass membrane protein.

• Sequence Similarities:

  Belongs to the peptidase S1 family. Plasminogen subfamily.

• Protein Name:

  Excitatory amino acid transporter 2