Anti-SPATA7 Antibody Picoband®, PE Conjugate

• Background:

  Spermatogenesis-associated protein 7 is a protein that in humans is encoded by the SPATA7 gene. This gene, originally isolated from testis, is also expressed in retina. Mutations in this gene are associated with Leber congenital amaurosis and juvenile retinitis pigmentosa. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

• Description:

  Boster Bio Anti-SPATA7 Antibody Picoband® catalog # A08310-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Histone-lysine N-methyltransferase SETDB2; Chronic lymphocytic leukemia deletion region gene 8 protein; Lysine N-methyltransferase 1F; SET domain bifurcated 2; SETDB2; C13orf4; CLLD8; KMT1F

• Gene Name:

  SET domain bifurcated histone lysine methyltransferase 2

• Gene ID:

  55812

• UniProt:

  Q9P0W8

• Host:

  Rabbit

• Reactivity:

  Human,Mouse,Rat

• Cross Reactivity:

  No cross-reactivity with other proteins.

• Immunogen:

  E.coli-derived human SPATA7 recombinant protein (Position: R5-V599).

• Clonality:

  Polyclonal

• Tissue Specificity:

  Highly expressed in reproductive organs, such as testis, ovary and prostate.

• Applications:

  WB,Flow Cytometry,ELISA

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Function:

  Histone methyltransferase involved in left-right axis specification in early development and mitosis. Specifically trimethylates 'Lys-9' of histone H3 (H3K9me3). H3K9me3 is a specific tag for epigenetic transcriptional repression that recruits HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Contributes to H3K9me3 in both the interspersed repetitive elements and centromere-associated repeats. Plays a role in chromosome condensation and segregation during mitosis.

• Components:

  Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

• References & Citations:

  1. Avila-Fernandez, A., Corton, M., Lopez-Molina, M. I., Martin-Garrido, E., Cantalapiedra, D., Fernandez-Sanchez, R., Blanco-Kelly, F., Riveiro-Alvarez, R., Tatu, S. D., Trujillo-Tiebas, M. J., Garcia-Sandoval, B., Ayuso, C., Cremers, F. P. M. Late onset retinitis pigmentosa. Ophthalmology 118: 2523-2524, 2011. 2. Eblimit, A., Agrawal, S. A., Thomas, K., Anastassov, I. A., Abulikemu, T., Moayedi, Y., Mardon, G., Chen, R. Conditional loss of Spata7 in photoreceptors causes progressive retinal degeneration in mice. Exp. Eye Res. 166: 120-130, 2018. Note: Erratum: Exp Eye Res. 171: 119, 2018. 3. Eblimit, A., Nguyen, T.-M. T., Chen, Y., Esteve-Rudd, J., Zhong, H., Letteboer, S., Van Reeuwijk, J., Simons, D. L., Ding, Q., Wu, K. M., Li, Y., Van Beersum, S., and 10 others. Spata7 is a retinal ciliopathy gene critical for correct RPGRIP1 localization and protein trafficking in the retina. Hum. Molec. Genet. 24: 1584-601, 2015.

• Storage Conditions:

  At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

• Observed Molecular Weight:

  68 kDa

• Fragment:

  Rabbit IgG

• Applications Notes:

  Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
  Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
  ELISA, 0.1-0.5 μg/ml, -
  

• Subcellular Location:

  Nucleus. Chromosome.