Anti-GIT2 Antibody Picoband®, PE Conjugate

Anti-GIT2 Antibody Picoband®, PE Conjugate

• Background: ARF GTPase-activating protein GIT2 is an enzyme that in humans is encoded by the GIT2 gene. This gene encodes a member of the GIT protein family, which interact with G protein-coupled receptor kinases and possess ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. GIT proteins traffic between cytoplasmic complexes, focal adhesions, and the cell periphery, and interact with Pak interacting exchange factor beta (PIX) to form large oligomeric complexes that transiently recruit other proteins. GIT proteins regulate cytoskeletal dynamics and participate in receptor internalization and membrane trafficking. This gene has been shown to repress lamellipodial extension and focal adhesion turnover, and is thought to regulate cell motility. This gene undergoes extensive alternative splicing to generate multiple isoforms, but the full-length nature of some of these variants has not been determined. The various isoforms have functional differences, with respect to ARF GAP activity and to G protein-coupled receptor kinase 2 binding.
• Description: Boster Bio Anti-GIT2 Antibody Picoband® catalog # A04441-3. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
• Synonyms: Interleukin-25; IL-25; Interleukin-17E; IL-17E; IL25; IL17E; UNQ3120/PRO10272
• Gene Name: interleukin 25
• Gene ID: 9815
• UniProt: Q14161
• Host: Rabbit
• Reactivity: Human,Mouse
• Cross Reactivity: No cross-reactivity with other proteins.
• Immunogen: E.coli-derived human GIT2 recombinant protein (Position: D400-S556).
• Clonality: Polyclonal
• Tissue Specificity: Expressed at low levels in several tissues, including brain, kidney, lung, prostate, testis, spinal cord, adrenal gland, and trachea.
• Applications: ELISA,Flow Cytometry,WB
• Purification: Immunogen affinity purified.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form: Lyophilized
• Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Function: Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. Proinflammatory cytokine favoring Th2-type immune responses.
• Components: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
• References & Citations: 1. Bagrodia, S., Bailey, D., Lenard, Z., Hart, M., Guan, J. L., Premont, R. T., Taylor, S. J., Cerione, R. A. A tyrosine-phosphorylated protein that binds to an important regulatory region on the Cool family of p21-activated kinase-binding proteins. J. Biol. Chem. 274: 22393-22400, 1999. 2. Mazaki, Y., Hashimoto, S., Okawa, K., Tsubouchi, A., Nakamura, K., Yagi, R., Yano, H., Kondo, A., Iwamatsu, A., Mizoguchi, A., Sabe, H. An ADP-ribosylation factor GTPase-activating protein Git2-short/KIAA0148 is involved in subcellular localization of paxillin and actin cytoskeletal organization. Molec. Biol. Cell 12: 645-662, 2001. 3. Nagase, T., Seki, N., Tanaka, A., Ishikawa, K., Nomura, N. Prediction of the coding sequences of unidentified human genes. IV. The coding sequences of 40 new genes (KIAA0121-KIAA0160) deduced by analysis of cDNA clones from human cell line KG-1. DNA Res. 2: 167-174, 1995.
• Storage Conditions: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.
• Observed Molecular Weight: 85 kDa
• Fragment: Rabbit IgG
• Applications Notes: Western blot, 0.1-0.25 μg/ml, Human, Mouse
  Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
  ELISA, 0.1-0.5 μg/ml, -
  
• Subcellular Location: Secreted.