Anti-Claudin 5/CLDN5 Antibody, PE Conjugate

• Background:

  Claudin-5 is a protein that in humans is encoded by the CLDN5 gene. This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Mutations in this gene have been found in patients with velocardiofacial syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

• Description:

  Boster Bio Anti-Claudin 5/CLDN5 Antibody catalog # A03260-3. Tested in IF, IHC applications. This antibody reacts with Human.

• Synonyms:

  Thrombospondin-2; THBS2; TSP2

• Gene Name:

  thrombospondin 2

• Gene ID:

  7122

• UniProt:

  O00501

• Host:

  Rabbit

• Reactivity:

  Human

• Cross Reactivity:

  No cross-reactivity with other proteins.

• Immunogen:

  A synthetic peptide corresponding to a sequence at the C-terminus of human Claudin 5/CLDN5, which shares 95% and 90% amino acid (aa) sequence identity with mouse and rat Claudin 5/CLDN5, respectively.

• Clonality:

  Polyclonal

• Tissue Specificity:

  High expression in invertebral disk tissue.

• Applications:

  IHC,IF

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

• Function:

  Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Ligand for CD36 mediating antiangiogenic properties.

• Components:

  Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

• References & Citations:

  1. Sirotkin, H., Morrow, B., Saint-Jore, B., Puech, A., Das Gupta, R., Patanjali, S. R., Skoultchi, A., Weissman, S. M., Kucherlapati, R. Identification, characterization, and precise mapping of a human gene encoding a novel membrane-spanning protein from the 22q11 region deleted in velo-cardio-facial syndrome. 2. Peacock RE, Keen TJ, Inglehearn CF (Mar 1998). "Analysis of a human gene homologous to rat ventral prostate.1 protein". Genomics 46 (3): 443–9. 3. "Entrez Gene: CLDN5 claudin 5 (transmembrane protein deleted in velocardiofacial syndrome)"

• Storage Conditions:

  Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

• Observed Molecular Weight:

  130 kDa

• Fragment:

  Rabbit IgG

• Applications Notes:

  Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human
  Immunofluorescence, 5μg/ml, Human
  

• Subcellular Location:

  Synapse. Postsynaptic density. Dendritic spine. Dendrite.