Anti-NPL4/NPLOC4 Antibody Picoband®, Dylight594 Conjugate

• Background:

  Nuclear protein localization protein 4 homolog is a protein that in humans is encoded by the NPLOC4 gene. Nuclear protein localization protein 4 (NPL4, NPLOC4) was originally identified as a yeast nuclear transport protein that was later recognized as a critical component of the endoplasmic reticulum-associated degradation (ERAD) pathway. Mammalian NPL4 protein has an amino-terminal ubiquitin-like domain containing a p97 binding site, and a conserved carboxy-terminal zinc finger (NZF) motif responsible for binding ubiquitinated target proteins. NPL4 binds ubiquitin fusion degradation protein 1 (UFD1) to form a heterodimer that associates with the p97 AAA-ATPase, creating a protein complex that mediates delivery of ubiquitinated ER proteins to the proteasome.

• Description:

  Boster Bio Anti-NPL4/NPLOC4 Antibody Picoband® catalog # A08476-1. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Serine/threonine-protein kinase LMTK3; Lemur tyrosine kinase 3; LMTK3; KIAA1883; TYKLM3

• Gene Name:

  lemur tyrosine kinase 3

• Gene ID:

  55666

• UniProt:

  Q8TAT6

• Host:

  Rabbit

• Reactivity:

  Human,Mouse,Rat

• Cross Reactivity:

  No cross-reactivity with other proteins.

• Immunogen:

  E.coli-derived human NPL4/NPLOC4 recombinant protein (Position: M1-N495).

• Clonality:

  Polyclonal

• Applications:

  WB,ICC,IF,ELISA

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Function:

  Protein kinase which phosphorylates ESR1 (in vitro) and protects it against proteasomal degradation. May also regulate ESR1 levels inly via a PKC-AKT-FOXO3 pathway where it decreases the activity of PKC and the phosphorylation of AKT, thereby increasing binding of transcriptional activator FOXO3 to the ESR1 promoter and increasing ESR1 transcription (PubMed:21602804). Involved in endocytic trafficking of N-methyl-D-aspartate receptors (NMDAR) in neurons.

• Components:

  Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

• References & Citations:

  1. Botta, A., Tandoi, C., Fini, G., Calabrese, G., Dallapiccola, B., Novelli, G. Cloning and characterization of the gene encoding human NPL4, a protein interacting with the ubiquitin fusion-degradation protein (UFD1L). Gene 275: 39-46, 2001. 2. Nagase, T., Kikuno, R., Ishikawa, K., Hirosawa, M., Ohara, O. Prediction of the coding sequences of unidentified human genes. XVII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. DNA Res. 7: 143-150, 2000.

• Storage Conditions:

  At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

• Observed Molecular Weight:

  72 kDa

• Fragment:

  Rabbit IgG

• Applications Notes:

  Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
  Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
  ELISA, 0.1-0.5 μg/ml, -
  

• Subcellular Location:

  Golgi apparatus membrane. Membrane. Single-pass membrane protein. Axon. Dendrite.