Anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 Antibody Picoband®, Dylight550 Conjugate

• Background:

  PDK1 (Pyruvate Dehydrogenase Kinase Isoenzyme 1), is an enzyme that in humans is encoded by the PDK1 gene. To find human PDKs, Gudi et al. (1995) used oligonucleotide primers to screen a human liver cDNA library by PCR. They identified and reported the deduced amino acid sequences of PDK1, PDK2, and PDK3. The human PDK1 gene encodes a protein with a predicted molecular mass of 49,244 Da that shares 93% identity with that of the rat PDK1 gene. Northern blot analysis showed that the PDK1 message was expressed predominantly in the heart.

• Description:

  Boster Bio Anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 Antibody catalog # PA1993. Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial;2.7.11.2;Pyruvate dehydrogenase kinase isoform 1;PDH kinase 1;PDK1;PDHK1;

• Gene Name:

  [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial

• UniProt:

  Q15118

• Host:

  Rabbit

• Reactivity:

  Human,Rat

• Cross Reactivity:

  No cross-reactivity with other proteins

• Immunogen:

  A synthetic peptide corresponding to a sequence at the C-terminus of human Mitochondrial Pyruvate dehydrogenase kinase 1, different from the related rat sequence by one amino acid, and from the related mouse sequence by two amino acids.

• Clonality:

  Polyclonal

• Tissue Specificity:

  Expressed predominantly in the heart. Detected at lower levels in liver, skeletal muscle and pancreas. .

• Applications:

  IHC,WB

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

• Function:

  Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress. .

• Components:

  Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

• References & Citations:

  1. Gudi, R., Bowker-Kinley, M. M., Kedishvili, N. Y., Zhao, Y., Popov, K. M. Diversity of the pyruvate dehydrogenase kinase gene family in humans. J. Biol. Chem. 270: 28989-28994, 1995.

• Storage Conditions:

  Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  49244 MW

• Observed Molecular Weight:

  85-91 kDa

• Fragment:

  Rabbit IgG

• Specificity:

  No cross reactivity with other proteins.

• Applications Notes:

  Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat
  Western blot, 0.1-0.5μg/ml, Human, Rat
  

• Subcellular Location:

  Mitochondrion matrix .

• Sequence Similarities:

  Belongs to the PDK/BCKDK protein kinase family.

• Protein Name:

  [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial