Anti-C18orf22/RBFA Antibody Picoband®, Dylight550 Conjugate

• Background:

  Predicted to be involved in rRNA processing. Predicted to be located in mitochondrion.

• Description:

  Boster Bio Anti-C18orf22/RBFA Antibody Picoband® catalog # A13257-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  RNA-binding protein 47;RNA-binding motif protein 47;RBM47;

• Gene Name:

  RNA-binding protein 47

• Gene ID:

  79863

• UniProt:

  Q8N0V3

• Host:

  Rabbit

• Reactivity:

  Human

• Cross Reactivity:

  No cross-reactivity with other proteins

• Immunogen:

  E.coli-derived human C18orf22/RBFA recombinant protein (Position: L15-D319).

• Clonality:

  Polyclonal

• Tissue Specificity:

  Abundantly expressed in tonsil, lymph node, and trachea; strong expression in prostate; lower expression in thyroid, stomach, and colon. .

• Applications:

  WB,IHC,ICC,IF,Flow Cytometry,ELISA

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Function:

  Can bind to the surface of B-lymphoma cells, but not T- lymphoma cells, consistent with a function as a secreted mediator acting upon B-cells.

• Components:

  Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

• References & Citations:

  1. Kuwasako, K. , Suzuki, S. , Nameki, N. , Takizawa, M. , Takahashi, M. , & Tsuda, K. , et al. (2022). 1h, 13c, and 15n resonance assignments and solution structures of the kh domain of human ribosome binding factor a, mtrbfa, involved in mitochondrial ribosome biogenesis. Biomolecular NMR Assignments, 1-7. 2. Rozanska, A. , Richter-Dennerlein, R. , Rorbach, J. , Gao, F. , & Lightowlers, R. N. . (2017). The human rna-binding protein rbfa promotes the maturation of the mitochondrial ribosome. Biochemical Journal, 474(13), 2145-2158. 3. Hauri, S. , Wepf, A. , Drogen, A. V. , Varjosalo, M. , & Gstaiger, M. . (2013). Interaction proteome of human hippo signaling: modular control of the co-activator yap1. Molecular Systems Biology, 9(1), 713.

• Storage Conditions:

  At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

• Calculated Molecular Weight:

  64099 MW

• Observed Molecular Weight:

  40 kDa

• Fragment:

  Rabbit IgG

• Applications Notes:

  Western blot, 0.25-0.5 μg/ml, Human
  Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
  Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
  Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
  ELISA, 0.1-0.5 μg/ml, -
  

• Subcellular Location:

  Nucleus .

• Protein Name:

  Follicular dendritic cell secreted peptide