Anti-Rab4/RAB4A Antibody Picoband®, Dylight550 Conjugate

• Background:

  This gene is a member of the largest group in the Ras superfamily of small GTPases, which regulate membrane trafficking. The encoded protein is associated with early endosomes and is involved in their sorting and recycling. The protein also plays a role in regulating the recycling of receptors from endosomes to the plasma membrane. Alternatively spliced transcript variants have been observed for this gene.

• Description:

  Boster Bio Anti-Rab4/RAB4A Antibody Picoband® catalog # A04643-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Solute carrier family 12 member 5; Electroneutral potassium-chloride cotransporter 2; K-Cl cotransporter 2; hKCC2; Neuronal K-Cl cotransporter; SLC12A5; KCC2; KIAA1176

• Gene Name:

  solute carrier family 12 member 5

• Gene ID:

  5867

• UniProt:

  P20338

• Host:

  Rabbit

• Reactivity:

  Human,Mouse,Rat

• Cross Reactivity:

  No cross-reactivity with other proteins.

• Immunogen:

  E.coli-derived human Rab4/RAB4A recombinant protein (Position: D110-C216).

• Clonality:

  Polyclonal

• Tissue Specificity:

  Brain specific. Detected in neuronal cells.

• Applications:

  WB,IHC,ELISA

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Function:

  Mediates electroneutral potassium-chloride cotransport in mature neurons and is required for neuronal Cl- homeostasis. As major extruder of intracellular chloride, it establishes the low neuronal Cl- levels required for chloride influx after binding of GABA-A and glycine to their receptors, with subsequent hyperpolarization and neuronal inhibition. Involved in the regulation of dendritic spine formation and maturation.

• Components:

  Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

• References & Citations:

  1. Barbosa, M. D. F. S., Johnson, S. A., Achey, K., Gutierrez, M., Wakeland, E. K., Zerial, M., Kingsmore, S. F. The Rab protein family: genetic mapping of six Rab genes in the mouse. Genomics 30: 439-444, 1995. 2. Bielli, A., Thornqvist, P.-O., Hendrick, A. G., Finn, R., Fitzgerald, K., McCaffrey, M. W. The small GTPase Rab4A interacts with the central region of cytoplasmic dynein light intermediate chain-1. Biochem. Biophys. Res. Commun. 281: 1141-1153, 2001. 3. Goueli, B. S., Powell, M. B., Finger, E. C., Pfeffer, S. R. TBC1D16 is a Rab4A GTPase activating protein that regulates receptor recycling and EGF receptor signaling. Proc. Nat. Acad. Sci. 109: 15787-15792, 2012.

• Storage Conditions:

  At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

• Observed Molecular Weight:

  24 kDa

• Fragment:

  Rabbit IgG

• Applications Notes:

  Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
  Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Rat
  ELISA, 0.1-0.5 μg/ml, -
  

• Subcellular Location:

  Membrane. Multi-pass membrane protein.