Anti-TTC11/FIS1 Antibody Picoband®, Dylight550 Conjugate

• Background:

  Mitochondrial fission 1 protein (FIS1) is a protein that in humans is encoded by the FIS1 gene on chromosome 7. It is mapped to 7q22.1. The balance between fission and fusion regulates the morphology of mitochondria. TTC11 is a component of a mitochondrial complex that promotes mitochondrial fission. Its role in mitochondrial fission thus implicates it in the regulation of mitochondrial morphology, the cell cycle, and apoptosis. By extension, the protein is involved in associated diseases, including neurodegenerative diseases and cancers.

• Description:

  Boster Bio Anti-TTC11/FIS1 Antibody catalog # A01932-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Mitochondrial fission 1 protein; FIS1 homolog; hFis1; Tetratricopeptide repeat protein 11; TPR repeat protein 11; FIS1; TTC11; CGI-135

• Gene Name:

  fission, mitochondrial 1

• Gene ID:

  51024

• UniProt:

  Q9Y3D6

• Host:

  Rabbit

• Reactivity:

  Human,Mouse,Rat

• Cross Reactivity:

  No cross-reactivity with other proteins.

• Immunogen:

  E.coli-derived human TTC11/FIS1 recombinant protein (Position: M1-D121).

• Clonality:

  Polyclonal

• Tissue Specificity:

  Ubiquitous. Expressed in the rod and cone photoreceptors, extending from the tips of the outer segment (OS) through the inner segment (IS) and outer nuclear layer (ONL) and into the synaptic terminals of the outer plexiform layer (ONL). Also detected in the bipolar, horizontal and amacrine cells in the inner nuclear layer (INL), extending to the inner plexiform layer (IPL) and though the ganglion cell layer (GCL) and into the nerve fiber layer (NFL) (at protein level).

• Applications:

  WB,IHC,ICC,IF,Flow Cytometry,ELISA

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

• Function:

  Involved in the fragmentation of the mitochondrial network and its perinuclear clustering. Plays a minor role in the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface and mitochondrial fission. Can induce cytochrome c release from the mitochondrion to the cytosol, ultimately leading to apoptosis. Also mediates peroxisomal fission.

• Components:

  Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

• References & Citations:

  1. Dohm, J. A., Lee, S. J., Hardwick, J. M., Hill, R. B., Gittis, A. G. Cytosolic domain of the human mitochondrial fission protein Fis1 adopts a TPR fold. Proteins 54: 153-156, 2004. 2. Frieden, M., James, D., Castelbou, C., Danckaert, A., Martinou, J.-C., Demaurex, N. Ca(2+) homeostasis during mitochondrial fragmentation and perinuclear clustering induced by hFis1. J. Biol. Chem. 279: 22704-22714, 2004. 3. James, D. I., Parone, P. A., Mattenberger, Y., Martinou, J.-C. hFis1, a novel component of the mammalian mitochondrial fission machinery. J. Biol. Chem. 278: 36373-36379, 2003. Note: Erratum: J. Biol. Chem. 279: 36166 only, 2004.

• Storage Conditions:

  Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

• Observed Molecular Weight:

  17 kDa

• Fragment:

  Rabbit IgG

• Specificity:

  No cross reactivity with other proteins.

• Applications Notes:

  Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
  Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
  Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
  Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
  ELISA, 0.1-0.5μg/ml, -
  

• Subcellular Location:

  Mitochondrion outer membrane. Single-pass membrane protein. Peroxisome membrane.