Anti-Cytochrome P450 2B6/CYP2B6 Antibody Picoband®, Dylight550 Conjugate

• Background:

  Cytochrome P450 2B6 is an enzyme that in humans is encoded by the CYP2B6 gene. It is mapped to 19q13.2. This gene, CYP2B6, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to metabolize some xenobiotics, such as the anti-cancer drugs cyclophosphamide and ifosphamide.

• Description:

  Boster Bio Anti-Cytochrome P450 2B6/CYP2B6 Antibody Picoband® catalog # A00861-2. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Cytochrome P450 2B6; 1,4-cineole 2-exo-monooxygenase; CYPIIB6; Cytochrome P450 IIB1; CYP2B6

• Gene Name:

  cytochrome P450 family 2 subfamily B member 6

• Gene ID:

  1555

• UniProt:

  P20813

• Host:

  Rabbit

• Reactivity:

  Human

• Cross Reactivity:

  No cross-reactivity with other proteins.

• Immunogen:

  A synthetic peptide corresponding to a sequence at the N-terminus of human Cytochrome P450 2B6/CYP2B6.

• Clonality:

  Polyclonal

• Tissue Specificity:

  Expressed in liver, lung and heart right ventricle.

• Applications:

  Flow Cytometry,IF,ICC,WB

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

• Function:

  Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Acts as a 1,4-cineole 2-exo-monooxygenase.

• Components:

  Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

• References & Citations:

  1. Blievernicht, J. K., Schaeffeler, E., Klein, K., Eichelbaum, M., Schwab, M., Zanger, U. M. MALDI-TOF mass spectrometry for multiplex genotyping of CYP2B6 single-nucleotide polymorphisms. Clin. Chem. 53: 24-33, 2007. 2. Carr, D. F., la Porte, C. J. L., Pirmohamed, M., Owen, A., Cortes, C. P. Haplotype structure of CYP2B6 and association with plasma efavirenz concentrations in a Chilean HIV cohort. J. Antimicrob. Chemother. 65: 1889-1893, 2010.

• Storage Conditions:

  Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

• Observed Molecular Weight:

  56 kDa

• Fragment:

  Rabbit IgG

• Specificity:

  No cross reactivity with other proteins.

• Applications Notes:

  Western blot, 0.1-0.5μg/ml
  Immunocytochemistry/Immunofluorescence, 5μg/ml
  Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells
  

• Subcellular Location:

  Endoplasmic reticulum membrane. Peripheral membrane protein. Microsome membrane. Peripheral membrane protein.