Anti-Rab9/RAB9A Antibody Picoband® (Dylight550 Conjugate)

• Background:

  Ras-related protein Rab-9A, also called RAB9, is a protein that in humans is encoded by the RAB9A gene. This gene is mapped to Xp22.2. RAB9 has been localized to components of the endocytic/exocytic pathway and has been implicated in recycling of membrane receptors. It has been found that downregulation of RAB9A gene expression in HeLa cells induced severe cell vacuolation. RAB9A GTPase is ly bound by TIP47 in its active, GTP-bound conformation. Moreover, RAB9A increases the affinity of TIP47 for its cargo. What’s more, this gene may involved in the transport of proteins between the endosomes and the trans Golgi network. RAB9A has been shown to interact with RABEPK and TIP47.

• Description:

  Boster Bio Anti-Rab9/RAB9A Antibody Picoband® catalog # PB9884. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Ras-related protein Rab-9A;RAB9A;RAB9;

• Gene Name:

  Ras-related protein Rab-9A

• UniProt:

  P51151

• Host:

  Rabbit

• Reactivity:

  Human,Monkey,Mouse

• Cross Reactivity:

  No cross-reactivity with other proteins.

• Immunogen:

  A synthetic peptide corresponding to a sequence at the C-terminus of human Rab9, different from the related mouse sequence by three amino acids, and from the related rat sequence by two amino acids.

• Clonality:

  Polyclonal

• Tissue Specificity:

  Widely expressed; high expression found in skeletal muscle. .

• Applications:

  Flow Cytometry,IP,IF,ICC,WB

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

• Function:

  Involved in the transport of proteins between the endosomes and the trans Golgi network.

• Storage Conditions:

  Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

• Fragment:

  Rabbit IgG

• Applications Notes:

  Western blot, 0.1-0.5μg/ml, Human, Monkey, Mouse
  Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
  Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
  

• Subcellular Location:

  Cell membrane ; Lipid-anchor ; Cytoplasmic side . Endoplasmic reticulum membrane . Golgi apparatus membrane . Late endosome. Cytoplasmic vesicle, phagosome membrane ; Lipid-anchor ; Cytoplasmic side . Cytoplasmic vesicle, phagosome. Colocalizes with OSBPL1A at the late endosome. Recruited to phagosomes containing S.aureus or M.tuberculosis.

• Protein Name:

  Ras-related protein Rab-9A