Anti-ERAB/HSD17B10 Antibody Picoband® (Dylight550 Conjugate)

• Background:

  ERAB is also known as HSD17B10 or HADH2. This gene encodes 3-hydroxyacyl-CoA dehydrogenase type II, a member of the short-chain dehydrogenase/reductase superfamily. The gene product is a mitochondrial protein that catalyzes the oxidation of a wide variety of fatty acids and steroids, and is a subunit of mitochondrial ribonuclease P, which is involved in tRNA maturation. The protein has been implicated in the development of Alzheimer disease, and mutations in the gene are the cause of 17beta-hydroxysteroid dehydrogenase type 10 (HSD10) deficiency. Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined.

• Description:

  Boster Bio Anti-ERAB/HSD17B10 Antibody Picoband® catalog # PB9336. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  3-hydroxyacyl-CoA dehydrogenase type-2;1.1.1.35;17-beta-hydroxysteroid dehydrogenase 10;17-beta-HSD 10;1.1.1.51;3-hydroxy-2-methylbutyryl-CoA dehydrogenase;1.1.1.178;3-hydroxyacyl-CoA dehydrogenase type II;Endoplasmic reticulum-associated amyloid beta-peptide-binding protein;Mitochondrial ribonuclease P protein 2;Mitochondrial RNase P protein 2;Short chain dehydrogenase/reductase family 5C member 1;Short-chain type dehydrogenase/reductase XH98G2;Type II HADH;HSD17B10;ERAB, HADH2, MRPP2, SCHAD, SDR5C1, XH98G2;

• Gene Name:

  3-hydroxyacyl-CoA dehydrogenase type-2

• UniProt:

  Q99714

• Host:

  Rabbit

• Reactivity:

  Human

• Cross Reactivity:

  No cross-reactivity with other proteins

• Immunogen:

  E.coli-derived human ERAB recombinant protein (Position: E48-P261). Human ERAB shares 87% and 88% amino acid (aa) sequence identity with mouse and rat ERAB, respectively.

• Clonality:

  Polyclonal

• Tissue Specificity:

  Ubiquitously expressed in normal tissues but is overexpressed in neurons affected in AD. .

• Applications:

  Flow Cytometry,IF,IHC,ICC,WB

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

• Function:

  Functions in mitochondrial tRNA maturation. Part of mitochondrial ribonuclease P, an enzyme composed of MRPP1/TRMT10C, MRPP2/HSD17B10 and MRPP3/KIAA0391, which cleaves tRNA molecules in their 5'-ends. Catalyzes the beta-oxidation at position 17 of androgens and estrogens and has 3-alpha-hydroxysteroid dehydrogenase activity with androsterone. Catalyzes the third step in the beta-oxidation of fatty acids. Carries out oxidative conversions of 7-alpha-OH and 7-beta-OH bile acids. Also exhibits 20-beta-OH and 21-OH dehydrogenase activities with C21 steroids. By interacting with intracellular amyloid-beta, it may contribute to the neuronal dysfunction associated with Alzheimer disease (AD). .

• Storage Conditions:

  Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

• Fragment:

  Rabbit IgG

• Applications Notes:

  Western blot, 0.1-0.5μg/ml
  Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, By Heat
  Immunocytochemistry/Immunofluorescence, 2μg/ml
  Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells
  

• Subcellular Location:

  Mitochondrion .

• Protein Name:

  3-hydroxyacyl-CoA dehydrogenase type-2