Anti-MCK10/NEP/DDR1 Antibody Picoband®, Dylight488 Conjugate

Anti-MCK10/NEP/DDR1 Antibody Picoband®, Dylight488 Conjugate

• Background: DDR1 (Discoidin domain receptor family, member 1) also known as NEP, EDDR1, NTRK4, TRKE, DDR, CAK or RTK6, is a human gene. The protein encoded by this gene is a RTK that is widely expressed in normal and transformed epithelial cells and is activated by various types of collagen.This protein belongs to a subfamily of tyrosine kinase receptors with a homology region to the Dictyostelium discoideum protein discoidin I in their extracellular domain.The DDR1 gene is mapped on 6p21.33. The fibrillar collagens and immobilized collagen activatedDDR1receptor phosphorylation after prolonged treatment. Bhatt et al. (2000)showed thatDdr1was highly expressed in the cerebellum of developing and adult mouse brain, and that bothDdr1and collagen IV were highly expressed in the pial layer of the cerebellar cortex.Cocultures of collagen I- and IV-expressing mouse pial cells withDdr1-expressing granule cells resulted in granule cell neurite extension. Inhibition of collagen-Ddr1signaling reduced granule cell neurite elongation.
• Description: Boster Bio Anti-MCK10/NEP/DDR1 Antibody Picoband® catalog # A00905. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
• Synonyms: Epithelial discoidin domain-containing receptor 1; Epithelial discoidin domain receptor 1; CD167 antigen-like family member A; Cell adhesion kinase; Discoidin receptor tyrosine kinase; HGK2; Mammary carcinoma kinase 10; MCK-10; Protein-tyrosine kinase 3A; Protein-tyrosine kinase RTK-6; TRK E; Tyrosine kinase DDR; Tyrosine-protein kinase CAK; CD167a; DDR1; CAK, EDDR1, NEP, NTRK4, PTK3A, RTK6, TRKE
• Gene Name: discoidin domain receptor tyrosine kinase 1
• Gene ID: 780
• UniProt: Q08345
• Host: Rabbit
• Reactivity: Human,Mouse,Rat
• Cross Reactivity: No cross-reactivity with other proteins.
• Immunogen: E.coli-derived human MCK10/NEP/DDR1 recombinant protein (Position: R341-A909).
• Clonality: Polyclonal
• Tissue Specificity: Detected in T-47D, MDA-MB-175 and HBL-100 breast carcinoma cells, A-431 epidermoid carcinoma cells, SW48 and SNU-C2B colon carcinoma cells and Hs 294T melanoma cells (at protein level). Expressed at low levels in most adult tissues and is highest in the brain, lung, placenta and kidney. Lower levels of expression are detected in melanocytes, heart, liver, skeletal muscle and pancreas. Abundant in breast carcinoma cell lines. In the colonic mucosa, expressed in epithelia but not in the connective tissue of the lamina propria. In the thyroid gland, expressed in the epithelium of the thyroid follicles. In pancreas, expressed in the islets of Langerhans cells, but not in the surrounding epithelial cells of the exocrine pancreas. In kidney, expressed in the epithelia of the distal tubules. Not expressed in connective tissue, endothelial cells, adipose tissue, muscle cells or cells of hematopoietic origin.
• Applications: ELISA,Flow Cytometry,IF,IHC,ICC,WB
• Purification: Immunogen affinity purified.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form: Lyophilized
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Function: Tyrosine kinase that functions as cell surface receptor for fibrillar collagen and regulates cell attachment to the extracellular matrix, remodeling of the extracellular matrix, cell migration, differentiation, survival and cell proliferation. Collagen binding triggers a signaling pathway that involves SRC and leads to the activation of MAP kinases. Regulates remodeling of the extracellular matrix by up-regulation of the matrix metalloproteinases MMP2, MMP7 and MMP9, and thereby facilitates cell migration and wound healing. Required for normal blastocyst implantation during pregnancy, for normal mammary gland differentiation and normal lactation. Required for normal ear morphology and normal hearing (By similarity). Promotes smooth muscle cell migration, and thereby contributes to arterial wound healing. Also plays a role in tumor cell invasion. Phosphorylates PTPN11.
• Components: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
• References & Citations: 1. Bhatt, R. S., Tomoda, T., Fang, Y., Hatten, M. E.Discoidin domain receptor 1 functions in axon extension of cerebellar granule neurons. Genes Dev. 14: 2216-2228, 2000. 2. Edelhoff, S., Sweetser, D. A., Disteche, C. M. Mapping of the NEP receptor tyrosine kinase gene to human chromosome 6p21.3 and mouse chromosome 17C. Genomics 25: 309-311, 1995. 3. Hou, G., Vogel, W., Bendeck, M. P. The discoidin domain receptor tyrosine kinase DDR1 in arterial wound repair. J. Clin. Invest. 107: 727-735, 2001.
• Storage Conditions: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
• Observed Molecular Weight: 101 kDa
• Fragment: Rabbit IgG
• Specificity: No cross reactivity with other proteins.
• Applications Notes: Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
  Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
  Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
  Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
  Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
  ELISA, 0.1-0.5μg/ml, -
  
• Subcellular Location: Cytoplasm.