Anti-14-3-3 sigma/SFN Antibody Picoband® (Dylight488 Conjugate)

• Background:

  Stratifin (SFN), also known as 14-3-3 protein sigma, is strongly induced by gamma irradiation and other DNA-damaging agents. The induction of 14-3-3-sigma is mediated by a p53 -responsive element located 1.8 kb upstream of its transcription start site. The protein, called stratifin, was shown to be diffusely distributed in the cytoplasm and was present in cultured epithelial cells. It was most abundant in tissues enriched in stratified keratinizing epithelium.

• Description:

  Boster Bio Anti-14-3-3 sigma/SFN Antibody Picoband® catalog # A01127. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  14-3-3 protein sigma; Epithelial cell marker protein 1; Stratifin; SFN; HME1

• Gene Name:

  stratifin

• UniProt:

  P31947

• Host:

  Rabbit

• Reactivity:

  Human,Mouse,Rat

• Cross Reactivity:

  No cross-reactivity with other proteins.

• Immunogen:

  E. coli-derived human 14-3-3 sigma recombinant protein (Position: M1-S248).

• Clonality:

  Polyclonal

• Tissue Specificity:

  Present mainly in tissues enriched in stratified squamous keratinizing epithelium.

• Applications:

  ELISA,Flow Cytometry,IF,IHC,ICC,WB

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

• Function:

  Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.

• Storage Conditions:

  Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

• Fragment:

  Rabbit IgG

• Applications Notes:

  Western blot, 0.1-0.5μg/ml
  Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
  Immunohistochemistry (Frozen Section), 0.5-1μg/ml
  Immunocytochemistry, 0.5-1μg/ml
  Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells
  Immunofluorescence, 2μg/ml
  ELISA, 0.1-0.5μg/ml
  

• Subcellular Location:

  Cytoplasm. Nucleus . Secreted. May be secreted by a non-classical secretory pathway.