Anti-LY6D Antibody Picoband®, Cy3 Conjugate

• Background:

  LY6D, also named as E48 and ThB, may act as a specification marker at earliest stage specification of lymphocytes between B- and T-cell development. It marks the earliest stage of B-cell specification.

• Description:

  Boster Bio Anti-LY6D Antibody Picoband® catalog # A09680. Tested in WB, IHC, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Gene Name:

  LY6D

• Gene ID:

  8581

• UniProt:

  Q14210

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  E.coli-derived human LY6D recombinant protein (Position: L21-L111). Human LY6D shares 60.4% amino acid (aa) sequence identity with mouse LY6D.

• Clonality:

  Polyclonal

• Applications:

  WB,IHC,ELISA

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Components:

  Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

• References & Citations:

  1. Brakenhoff, R. H., Gerretsen, M., Knippels, E. M. C., van Dijk, M., van Essen, H., Weghuis, D. O., Sinke, R. J., Snow, G. B., van Dongen, G. A. M. S. The human E48 antigen, highly homologous to the murine Ly-6 antigen ThB, is a GPI-anchored molecule apparently involved in keratinocyte cell-cell adhesion. J. Cell Biol. 129: 1677-1689, 1995. 2. Brakenhoff, R. H., van Dijk, M., Rood-Knippels, E. M. C., Snow, G. B. A gain of novel tissue specificity in the human Ly-6 gene E48. J. Immun. 159: 4879-4886, 1997. 3. Inlay, M. A., Bhattacharya, D., Sahoo, D., Serwold, T., Seita, J., Karsunky, H., Plevritis, S. K., Dill, D. L., Weissman, I. L. Ly6d marks the earliest stage of B-cell specification and identifies the branchpoint between B-cell and T-cell development. Genes Dev. 23: 2376-2381, 2009. Note: Erratum: Genes Dev. 27: 2063 only, 2013.

• Storage Conditions:

  At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

• Observed Molecular Weight:

  16 kDa

• Applications Notes:

  Western blot, 0.1-0.25 μg/ml, Human
  Immunohistochemistry, 2-5 μg/ml, Human
  ELISA, 0.1-0.5 μg/ml, -