Anti-RTN4IP1 Antibody Picoband®, Cy3 Conjugate

• Background:

  This gene encodes a mitochondrial protein that interacts with reticulon 4, which is a potent inhibitor of regeneration following spinal cord injury. This interaction may be important for reticulon-induced inhibition of neurite growth. Mutations in this gene can cause optic atrophy 10, with or without ataxia, cognitive disability, and seizures. There is a pseudogene for this gene on chromosome 12. Alternative splicing results in multiple transcript variants.

• Description:

  Boster Bio Anti-RTN4IP1 Antibody Picoband® catalog # A13135-1. Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Follicular dendritic cell secreted peptide;FDC secreted protein;FDC-SP;FDCSP;C4orf7;UNQ733/PRO1419;

• Gene Name:

  Follicular dendritic cell secreted peptide

• Gene ID:

  84816

• UniProt:

  Q8WWV3

• Host:

  Rabbit

• Reactivity:

  Human,Mouse,Rat

• Cross Reactivity:

  No cross-reactivity with other proteins

• Immunogen:

  E.coli-derived human RTN4IP1 recombinant protein (Position: M1-K381).

• Clonality:

  Polyclonal

• Tissue Specificity:

  Abundantly expressed in tonsil, lymph node, and trachea; strong expression in prostate; lower expression in thyroid, stomach, and colon. .

• Applications:

  ELISA,WB,Flow Cytometry

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Function:

  Can bind to the surface of B-lymphoma cells, but not T- lymphoma cells, consistent with a function as a secreted mediator acting upon B-cells.

• Components:

  Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

• References & Citations:

  1. Angebault, C., Guichet, P.-O., Talmat-Amar, Y., Charif, M., Gerber, S., Fares-Taie, L., Gueguen, N., Halloy, F., Moore, D., Amati-Bonneau, P., Manes, G., Hebrard, M., and 21 others. Recessive mutations in RTN4IP1 cause isolated and syndromic optic neuropathies. Am. J. Hum. Genet. 97: 754-760, 2015. Note: Erratum: Am. J. Hum. Genet. 97: 769 only, 2015. 2. Charif, M., Nasca, A., Thompson, K., Gerber, S., Makowski, C., Mazaheri, N., Bris, C., Goudenege, D., Legati, A., Maroofian, R., Shariati, G., Lamantea, E., and 30 others. Neurologic phenotypes associated with mutations in RTN4IP1 (OPA10) in children and young adults. JAMA Neurol. 75: 105-113, 2018. 3. Hartz, P. A. Personal Communication. Baltimore, Md. 10/28/2010.

• Storage Conditions:

  At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

• Calculated Molecular Weight:

  9700 MW

• Observed Molecular Weight:

  44 kDa

• Fragment:

  Rabbit IgG

• Applications Notes:

  Western blot, 0.25-0.5 μg/ml, Mouse, Rat
  Flow Cytometry (Fixed), 1-3 μg /1x10⁶ cells, Human
  ELISA, 0.1-0.5 μg/ml, -
  

• Subcellular Location:

  Secreted.

• Protein Name:

  Follicular dendritic cell secreted peptide