Anti-Spermidine synthase/SRM Antibody Picoband®, Cy3 Conjugate

• Background:

  The polyamines putrescine, spermine, and spermidine are ubiquitous polycationic mediators of cell growth and differentiation. Spermidine synthase is one of four enzymes in the polyamine-biosynthetic pathway and carries out the final step of spermidine biosynthesis. This enzyme catalyzes the conversion of putrescine to spermidine using decarboxylated S-adenosylmethionine as the cofactor.

• Description:

  Boster Bio Anti-Spermidine synthase/SRM Antibody Picoband® catalog # A01594-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Neutrophil cytosol factor 1; NCF-1; 47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NCF-47K; Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox-organizing protein 2; SH3 and PX domain-containing protein 1A; p47-phox; NCF1; NOXO2; SH3PXD1A

• Gene Name:

  neutrophil cytosolic factor 1

• Gene ID:

  6723

• UniProt:

  P19623

• Host:

  Rabbit

• Reactivity:

  Human

• Cross Reactivity:

  No cross-reactivity with other proteins.

• Immunogen:

  E.coli-derived human Spermidine synthase/SRM recombinant protein (Position: A15-E292).

• Clonality:

  Polyclonal

• Tissue Specificity:

  Detected in peripheral blood monocytes and neutrophils (at protein level).

• Applications:

  WB,Flow Cytometry,ELISA

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Function:

  NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).

• Components:

  Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

• References & Citations:

  1. Myohanen, S., Kauppinen, L., Wahlfors, J., Alhonen, L., Janne, J. Human spermidine synthase gene: structure and chromosomal localization. DNA Cell Biol. 10: 467-474, 1991. 2. Wahlfors, J., Alhonen, L., Kauppinen, L., Hyvonen, T., Janne, J., Eloranta, T. O. Human spermidine synthase: cloning and primary structure. DNA Cell Biol. 9: 103-110, 1990. Note: Erratum: DNA Cell Biol. 9: 543 only, 1990. 3. Winqvist, R., Alanen, L., Grzeschik, K.-H., Janne, J., Eloranta, T. Mapping of DNA sequences for spermidine synthase to human chromosome 1p36-p22 and chromosome 3p14-q21. (Abstract) Cytogenet. Cell Genet. 58: 1865 only, 1991.

• Storage Conditions:

  At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

• Calculated Molecular Weight:

  34140 MW

• Observed Molecular Weight:

  34 kDa

• Fragment:

  Rabbit IgG

• Applications Notes:

  Western blot, 0.25-0.5 μg/ml, Human
  Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
  ELISA, 0.1-0.5 μg/ml, -
  

• Subcellular Location:

  Cytoplasm, cytosol.

• Protein Name:

  Growth/differentiation factor 15