Anti-CRMP4/Dpysl3 Antibody Picoband®, Cy3 Conjugate

• Background:

  Dihydropyrimidinase-related protein 3 is an enzyme that in humans is encoded by the DPYSL3 gene. This gene encodes a protein that belongs to the TUC (TOAD-64/Ulip/CRMP) family of proteins. Members of this family are phosphoproteins that function in axonal guidance and neuronal differentiation during development and regeneration of the nervous system. A mutation in the human gene is associated with amyotrophic lateral sclerosis. Alternative splicing results in multiple transcript variants encoding different isoforms.

• Description:

  Boster Bio Anti-CRMP4/Dpysl3 Antibody Picoband® catalog # A05960-1. Tested in WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Long-chain-fatty-acid--CoA ligase 3;6.2.1.3;Long-chain acyl-CoA synthetase 3;LACS 3;ACSL3;ACS3, FACL3, LACS3;

• Gene Name:

  Long-chain-fatty-acid--CoA ligase 3

• Gene ID:

  25418

• UniProt:

  Q62952

• Host:

  Rabbit

• Reactivity:

  Rat

• Cross Reactivity:

  No cross-reactivity with other proteins.

• Immunogen:

  A synthetic peptide corresponding to a sequence at the C-terminus of rat CRMP4/Dpysl3, which shares 93.3% amino acid (aa) sequence identity with both human and mouse CRMP4/Dpysl3.

• Clonality:

  Polyclonal

• Tissue Specificity:

  Expressed in breast, ductal and invasive ductal carcinomas of the breast, sporadic colorectal adenomas and carcinomas (at protein level). Expressed in fetal brain. Expressed in lung, amygdala, eye, prostate, pancreatic and prostate cancers, head and neck tumors and embryonal tumor.

• Applications:

  WB

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Function:

  Acyl-CoA synthetases (ACSL) activates long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL3 mediates hepatic lipogenesis (By similarity). Preferentially uses myristate, laurate, arachidonate and eicosapentaenoate as substrates (By similarity). Has mainly an anabolic role in energy metabolism. Required for the incorporation of fatty acids into phosphatidylcholine, the major phospholipid located on the surface of VLDL (very low density lipoproteins). .

• Components:

  Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

• References & Citations:

  1. Blasco, H., Bernard-Marissal, N., Vourc'h, P., Guettard, Y. O., Sunyach, C., Augereau, O., Khederchah, J., Mouzat, K., Antar, C., Gordon, P. H., Veyrat-Durebex, C., Besson, G., Andersen, P. M., Salachas, F., Meininger, V., Camu, W., Pettmann, B., Andres, C. R., Corcia, P., the French ALS Study Group. A rare motor neuron deleterious missense mutation in the DPYSL3 (CRMP4) gene is associated with ALS. Hum. Mutat. 34: 953-960, 2013. 2. Byk, T., Dobransky, T., Cifuentes-Diaz, C., Sobel, A. Identification and molecular characterization of Unc-33-like phosphoprotein (Ulip), a putative mammalian homolog of the axonal guidance-associated unc-33 gene product. J. Neurosci. 16: 688-701, 1996. 3. Gaetano, C, Matsuo, T., Thiele, C. J. Identification and characterization of a retinoic acid-regulated human homologue of the unc-33-like phosphoprotein gene (hUlip) from neuroblastoma cells. J. Biol. Chem. 272: 12195-12201, 1997.

• Storage Conditions:

  At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

• Calculated Molecular Weight:

  80420 MW

• Observed Molecular Weight:

  62 kDa

• Fragment:

  Rabbit IgG

• Applications Notes:

  Western blot, 0.25-0.5 μg/ml, Rat
  

• Subcellular Location:

  Mitochondrion outer membrane ; Single-pass type III membrane protein . Peroxisome membrane ; Single-pass type III membrane protein . Microsome membrane ; Single-pass type III membrane protein . Endoplasmic reticulum membrane ; Single-pass type III membrane protein .

• Protein Name:

  Long-chain-fatty-acid--CoA ligase 3