Anti-Cyclin A1/CCNA1 Antibody Picoband®, Cy3 Conjugate

Anti-Cyclin A1/CCNA1 Antibody Picoband®, Cy3 Conjugate

• Background: Cyclin-A1 is a protein that in humans is encoded by the CCNA1 gene. The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. The cyclin encoded by this gene was shown to be expressed in testis and brain, as well as in several leukemic cell lines, and is thought to primarily function in the control of the germline meiotic cell cycle. This cyclin binds both CDK2 and CDC2 kinases, which give two distinct kinase activities, one appearing in S phase, the other in G2, and thus regulate separate functions in cell cycle. Also this cyclin was found to bind to important cell cycle regulators, such as Rb family proteins, transcription factor E2F-1, and the p21 family proteins. Multiple transcript variants encoding different isoforms have been found for this gene.
• Description: Boster Bio Anti-Cyclin A1/CCNA1 Antibody Picoband® catalog # PB9485. Tested in IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
• Synonyms: Cyclin-A1;CCNA1;
• Gene Name: Cyclin-A1
• Gene ID: 8900
• UniProt: P78396
• Host: Rabbit
• Reactivity: Human
• Cross Reactivity: No cross-reactivity with other proteins
• Immunogen: E.coli-derived human Cyclin A1 recombinant protein (Position: D64-E263). Human Cyclin A1 shares 78.5% and 79.5% amino acid (aa) sequence identity with mouse and rat Cyclin A1, respectively.
• Clonality: Polyclonal
• Tissue Specificity: Very high levels in testis and very low levels in brain. Also found in myeloid leukemia cell lines.
• Applications: IF,ICC,WB
• Purification: Immunogen affinity purified.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form: Lyophilized
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Function: May be involved in the control of the cell cycle at the G1/S (start) and G2/M (mitosis) transitions. May primarily function in the control of the germline meiotic cell cycle and additionally in the control of mitotic cell cycle in some somatic cells. .
• Components: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2mg Na2HPO4, 0.05 mg NaN3.
• References & Citations: 1. "Entrez Gene: CCNA1 cyclin A1". 2. Yang R, Morosetti R, Koeffler HP (March 1997). "Characterization of a second human cyclin A that is highly expressed in testis and in several leukemic cell lines".Cancer Res. 57 (5): 913–20.
• Storage Conditions: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
• Calculated Molecular Weight: 52358 MW
• Observed Molecular Weight: 55 kDa
• Fragment: Rabbit IgG
• Specificity: No cross reactivity with other proteins.
• Applications Notes: Western blot, 0.1-0.5μg/ml, Human
  Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
  
• Subcellular Location: Nucleus .
• Sequence Similarities: Belongs to the cyclin family. Cyclin AB subfamily.
• Protein Name: Cyclin-A1