Anti-LDL Receptor/LDLR Antibody Picoband® (Biotine Conjugate)

• Background:

  In humans, the LDL receptor protein is encoded by the LDLR gene on chromosome 19. It is mapped to 19p13.2. The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. Alternate splicing results in multiple transcript variants.

• Description:

  Boster Bio Anti-LDL Receptor/LDLR Antibody Picoband® catalog # A00076-2. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Low-density lipoprotein receptor; LDL receptor; LDLR

• Gene Name:

  low density lipoprotein receptor

• UniProt:

  P01130

• Host:

  Rabbit

• Reactivity:

  Human,Rat

• Cross Reactivity:

  No cross-reactivity with other proteins.

• Immunogen:

  E.coli-derived human LDL Receptor/LDLR recombinant protein (Position: Q35-D843).

• Clonality:

  Polyclonal

• Tissue Specificity:

  Widely expressed, including in adult and fetal brain, placenta, skin fibroblasts, adipose tissue and gonads.

• Applications:

  WB,IHC,Flow Cytometry,ELISA

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

• Function:

  Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. (Microbial infection) Acts as a receptor for hepatitis C virus in hepatocytes, but not through a interaction with viral proteins. (Microbial infection) Acts as a receptor for Vesicular stomatitis virus. (Microbial infection) In case of HIV-1 infection, may function as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells.

• Storage Conditions:

  Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

• Fragment:

  Rabbit IgG

• Applications Notes:

  Western blot, 0.25-0.5μg/ml, Human, Rat
  Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
  Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human, Rat
  ELISA, 0.1-0.5μg/ml, -
  

• Subcellular Location:

  Lysosome. Golgi apparatus. Cell membrane. Single-pass type I membrane protein. Early endosome. Late endosome. Clathrin-coated pit.