Anti-NRAMP1/SLC11A1 Antibody Picoband® (Biotine Conjugate)

• Background:

  Natural resistance-associated macrophage protein 1 is a protein that in humans is encoded by the SLC11A1 gene. It is mapped to 2q35. This gene is a member of the solute carrier family 11 (proton-coupled divalent metal ion transporters) family and encodes a multi-pass membrane protein. The protein functions as a divalent transition metal (iron and manganese) transporter involved in iron metabolism and host resistance to certain pathogens. Mutations in this gene have been associated with susceptibility to infectious diseases such as tuberculosis and leprosy, and inflammatory diseases such as rheumatoid arthritis and Crohn disease. Alternatively spliced variants that encode different protein isoforms have been described but the full-length nature of only one has been determined.

• Description:

  Boster Bio Anti-NRAMP1/SLC11A1 Antibody Picoband® catalog # A02547-3. Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Natural resistance-associated macrophage protein 1; NRAMP 1; Solute carrier family 11 member 1; SLC11A1; LSH; NRAMP; NRAMP1

• Gene Name:

  solute carrier family 11 member 1

• UniProt:

  P49279

• Host:

  Rabbit

• Reactivity:

  Human,Mouse,Rat

• Cross Reactivity:

  No cross-reactivity with other proteins.

• Immunogen:

  E.coli-derived human NRAMP1/SLC11A1 recombinant protein (Position: F183-S549).

• Clonality:

  Polyclonal

• Tissue Specificity:

  Macrophages; peripheral blood leukocytes, lung, spleen and liver.

• Applications:

  ELISA,IF,IHC,ICC,WB

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

• Function:

  Divalent transition metal (iron and manganese) transporter involved in iron metabolism and host resistance to certain pathogens. Macrophage-specific membrane transport function. Controls natural resistance to infection with intracellular parasites. Pathogen resistance involves sequestration of Fe2+ and Mn2+, cofactors of both prokaryotic and eukaryotic catalases and superoxide dismutases, not only to protect the macrophage against its own generation of reactive oxygen species, but to deny the cations to the pathogen for synthesis of its protective enzymes.

• Storage Conditions:

  Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

• Fragment:

  Rabbit IgG

• Applications Notes:

  Western blot, 0.1-0.5μg/ml
  Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
  Immunocytochemistry/Immunofluorescence, 2μg/ml
  ELISA, 0.1-0.5μg/ml
  

• Subcellular Location:

  Membrane. Multi-pass membrane protein.