Anti-RUNX1T1/ETO Antibody Picoband®, Biotin Conjugate

Anti-RUNX1T1/ETO Antibody Picoband®, Biotin Conjugate

• Background: Protein CBFA2T1 is a protein that in humans is encoded by the RUNX1T1 gene. This gene encodes a member of the myeloid translocation gene family which interact with DNA-bound transcription factors and recruit a range of corepressors to facilitate transcriptional repression. The t (8;21) (q22;q22) translocation is one of the most frequent karyotypic abnormalities in acute myeloid leukemia. The translocation produces a chimeric gene made up of the 5'-region of the runt-related transcription factor 1 gene fused to the 3'-region of this gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. Alternative splicing results in multiple transcript variants.
• Description: Boster Bio Anti-RUNX1T1/ETO Antibody Picoband® catalog # A01792-1. Tested in ELISA, Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
• Synonyms: Protein CBFA2T1; Cyclin-D-related protein; Eight twenty one protein; Protein ETO; Protein MTG8; Zinc finger MYND domain-containing protein 2; RUNX1T1; AML1T1; CBFA2T1; CDR; ETO; MTG8; ZMYND2
• Gene Name: RUNX1 translocation partner 1
• Gene ID: 862
• UniProt: Q06455
• Host: Rabbit
• Reactivity: Human,Mouse,Rat
• Cross Reactivity: No cross-reactivity with other proteins.
• Immunogen: E. coli-derived human RUNX1T1/ETO recombinant protein (Position: T335-D510).
• Clonality: Polyclonal
• Tissue Specificity: Most abundantly expressed in brain. Lower levels in lung, heart, testis and ovary.
• Applications: ELISA,Flow Cytometry,IF,IHC,IHC-F,ICC,WB
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form: Lyophilized
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Function: Transcriptional corepressor which facilitates transcriptional repression via its association with DNA-binding transcription factors and recruitment of other corepressors and histone-modifying enzymes (PubMed:12559562, PubMed:15203199). Can repress the expression of MMP7 in a ZBTB33-dependent manner (PubMed:23251453). Can repress transactivation mediated by TCF12 (PubMed:16803958). Acts as a negative regulator of adipogenesis (By similarity). The AML1-MTG8/ETO fusion protein frequently found in leukemic cells is involved in leukemogenesis and contributes to hematopoietic stem/progenitor cell self-renewal (PubMed:23812588).
• Components: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.
• References & Citations: 1. "Entrez Gene: RUNX1T1 runt-related transcription factor 1; translocated to, 1 (cyclin D-related)". 2. Calabi F, Cilli V (Dec 1998). "CBFA2T1, a gene rearranged in human leukemia, is a member of a multigene family". Genomics. 52 (3): 332–341.
• Storage Conditions: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
• Observed Molecular Weight: 67 kDa
• Fragment: Rabbit IgG
• Specificity: No cross reactivity with other proteins.
• Applications Notes: Western blot, 0.1-0.5μg/ml
  Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
  Immunohistochemistry (Frozen Section), 0.5-1μg/ml
  Immunocytochemistry/Immunofluorescence, 2μg/ml
  Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells
  ELISA, 0.1-0.5μg/ml
  
• Subcellular Location: Nucleus.