Anti-PRMT4/CARM1 Antibody Picoband®

• Background:

  CARM1 (coactivator-associated arginine methyltransferase 1), also known as PRMT4, is an enzyme encoded by the CARM1 gene found in human beings, as well as many other mammals. This gene is mapped to 19p13.2. CARM1 is a regulator of cyclin E1 and DHFR mRNA expression. Its main function includes catalyzing the transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. CARM1 is a secondary coactivator through its association with p160 family (SRC-1, GRIP1, AIB) of coactivators. It is responsible for moving cells toward the inner cell mass in developing blastocysts. This gene also plays an important role in androgen receptors and may play a role in prostate cancer progression.

• Description:

  Boster Bio Anti-PRMT4/CARM1 Antibody catalog # PA2263. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Histone-arginine methyltransferase CARM1;2.1.1.-;2.1.1.125; Coactivator-associated arginine methyltransferase 1; Protein arginine N-methyltransferase 4; CARM1; PRMT4

• Gene Name:

  Histone-arginine methyltransferase CARM1

• UniProt:

  Q86X55

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Cross Reactivity:

  No cross-reactivity with other proteins

• Immunogen:

  A synthetic peptide corresponding to a sequence in the middle region of human PRMT4, identical to the related mouse and rat sequences.

• Clonality:

  Polyclonal

• Tissue Specificity:

  Overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia. .

• Applications:

  WB

• Field of Research:

  Cancer, Cancer Metabolism, Cell Biology, Cell Cycle, Cell Cycle Inhibitors, Chromatin Modifying Enzymes, Co-Activators/Co-Repressors, Co-Factors, Cofactors, Vitamins/Minerals, Epigenetics and Nuclear Signaling, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism of Lipids and Lipoproteins, Methylation, Nuclear Hormone Receptors, Nuclear Signaling, Oncoproteins/Suppressors, p53 Pathway, Pathways and Processes, Signal Transduction, Signaling Pathway, Transcription, Tumor Suppressors

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

• Function:

  Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activate transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Acts as coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue. Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at 'Arg- 2142', which may loosen its interaction with NCOA2/GRIP1, and at 'Arg-580' and 'Arg-604' in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA- stabilizing properties and the half-life of their target mRNAs. .

• Storage Conditions:

  Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

• Fragment:

  Rabbit IgG

• Applications Notes:

  Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
  

• Other Gene Names:

  CARM1

• Subcellular Location:

  Nucleus. Cytoplasm. Mainly nuclear during the G1, S and G2 phases of the cell cycle. Cytoplasmic during mitosis, after breakup of the nuclear membrane.

• Sequence Similarities:

  Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N- methyltransferase family.

• Protein Name:

  Histone-arginine methyltransferase CARM1