Anti-NT5C1A Antibody Picoband®

• Background:

  5'-nucleotidase, cytosolic IA is a protein that in humans is encoded by the NT5C1A gene. Cytosolic nucleotidases, such as NT5C1A, dephosphorylate nucleoside monophosphates.

• Description:

  Boster Bio Anti-NT5C1A Antibody Picoband® catalog # A12036-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Negative elongation factor B; NELF-B; Cofactor of BRCA1; NELFB; COBRA1; KIAA1182

• Gene Name:

  Negative elongation factor complex member B

• UniProt:

  Q9BXI3

• Host:

  Rabbit

• Reactivity:

  Human

• Cross Reactivity:

  No cross-reactivity with other proteins.

• Immunogen:

  E.coli-derived human NT5C1A recombinant protein (Position: E2-Q368) .

• Clonality:

  Polyclonal

• Tissue Specificity:

  Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal muscle, kidney and pancreas.

• Applications:

  WB, IHC, ELISA

• Field of Research:

  Epigenetics and Nuclear Signaling, Transcription

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Function:

  Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II. Binds RNA which may help to stabilize the NELF complex on nucleic acid. In vitro, binds weakly to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1. May be able to induce chromatin unfolding.

• Storage Conditions:

  At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

• Fragment:

  Rabbit IgG

• Applications Notes:

  Western blot, 0.25-0.5 μg/ml, Human
  Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
  ELISA, 0.1-0.5 μg/ml, -
  

• Other Gene Names:

  NT5C1A

• Subcellular Location:

  Nucleus.