Anti-COL4A3 Antibody

• Background:

  Collagen alpha-3 (IV) chain is a protein that in humans is encoded by the COL4A3 gene. Type IV collagen, the major structural component of basement membranes, is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. This gene encodes alpha 3. In the Goodpasture syndrome, autoantibodies bind to the collagen molecules in the basement membranes of alveoli and glomeruli. The epitopes that elicit these autoantibodies are localized largely to the non-collagenous C-terminal domain of the protein. A specific kinase phosphorylates amino acids in this same C-terminal region and the expression of this kinase is upregulated during pathogenesis. This gene is also linked to an autosomal recessive form of Alport syndrome. The mutations contributing to this syndrome are also located within the exons that encode this C-terminal region. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter.

• Description:

  Boster Bio Anti-COL4A3 Antibody Picoband® catalog # A01755-3. Tested in ELISA, IHC applications. This antibody reacts with Human.

• Synonyms:

  Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial; Flavoprotein subunit of complex II; Fp; SDHA; SDH2; SDHF

• Gene Name:

  Succinate dehydrogenase complex flavoprotein subunit A

• UniProt:

  Q01955

• Host:

  Rabbit

• Reactivity:

  Human

• Cross Reactivity:

  No cross-reactivity with other proteins.

• Immunogen:

  E.coli-derived human COL4A3 recombinant protein (Position: P1426-H1670) .

• Clonality:

  Polyclonal

• Tissue Specificity:

  Expressed in adipose tissue, heart, brain, skeletal muscle, pancreas, thymus, prostate, testis, ovary, small intestine and colon. Highest levels in adipose tissue, small intestine and colon.

• Applications:

  IHC, ELISA

• Field of Research:

  Amino Acid Metabolism, Amino Acids, Cancer, Energy Metabolism, Energy Transfer Pathways, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Signal Transduction

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Function:

  Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) . Can act as a tumor suppressor.

• Storage Conditions:

  At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

• Fragment:

  Rabbit IgG

• Applications Notes:

  Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
  ELISA, 0.1-0.5 μg/ml, -
  

• Other Gene Names:

  COL4A3

• Subcellular Location:

  Mitochondrion inner membrane. Peripheral membrane protein. Matrix side.