Anti-Cytochrome P450 2A6/CYP2A6 Antibody Picoband®

• Background:

  Cytochrome P450 2A6 (abbreviated CYP2A6) is a member of the cytochrome P450 mixed-function oxidase system, which is involved in the metabolism of xenobiotics in the body. CYP2A6 is the primary enzyme responsible for the oxidation of nicotine and cotinine. It is also involved in the metabolism of several pharmaceuticals, carcinogens, and a number of coumarin-type alkaloids. CYP2A6 is the only enzyme in the human body that appreciably catalyzes the 7-hydroxylation of coumarin, such that the formation of the product of this reaction, 7-hydroxycoumarin, is used as a probe for CYP2A6 activity. The CYP2A6 gene is part of a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q. The gene was formerly referred to as CYP2A3; however, it has been renamed CYP2A6.

• Description:

  Boster Bio Anti-Cytochrome P450 2A6/CYP2A6 Antibody Picoband® catalog # A00947-2. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Cytochrome P450 2A6; 1,4-cineole 2-exo-monooxygenase; CYPIIA6; Coumarin 7-hydroxylase; Cytochrome P450 IIA3; Cytochrome P450 (I) ; CYP2A6; CYP2A3

• Gene Name:

  Cytochrome P450 family 2 subfamily A member 6

• UniProt:

  P11509

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Cross Reactivity:

  No cross-reactivity with other proteins.

• Immunogen:

  A synthetic peptide corresponding to a sequence at the N-terminus of human Cytochrome P450 2A6/CYP2A6.

• Clonality:

  Polyclonal

• Tissue Specificity:

  Liver.

• Applications:

  WB

• Field of Research:

  Cell Differentiation, Developmental Biology, Epigenetics and Nuclear Signaling, Hematopoietic Progenitors, Organogenesis, Stem Cells

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

• Function:

  Exhibits a high coumarin 7-hydroxylase activity. Can act in the hydroxylation of the anti-cancer drugs cyclophosphamide and ifosphamide. Competent in the metabolic activation of aflatoxin B1. Constitutes the major nicotine C-oxidase. Acts as a 1,4-cineole 2-exo-monooxygenase. Possesses low phenacetin O-deethylation activity.

• Storage Conditions:

  Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

• Fragment:

  Rabbit IgG

• Applications Notes:

  Western blot, 0.1-0.5μg/ml
  

• Other Gene Names:

  CYP2A6

• Subcellular Location:

  Endoplasmic reticulum membrane. Peripheral membrane protein. Microsome membrane. Peripheral membrane protein

• Protein Name:

  Protein C-ets-1