Anti-Lipocalin 2/LCN2 Antibody

• Background:

  Europhile gelatinase-associated lipocalin (NGAL) is a protein that in humans is encoded by the LCN2 gene. The binding of lipocalin-2 to bacterial siderophores is important in the innate immune response to bacterial infection. Upon encountering invading bacteria the toll-like receptors on immune cells stimulate the synthesis and secretion of lipocalin-2. Secreted lipocalin-2 then limits bacterial growth by sequestering iron-containing siderophores. Lipocalin-2 also functions as a growth factor.

• Description:

  Anti-Lipocalin 2/LCN2 Antibody

• Synonyms:

  Neutrophil gelatinase-associated lipocalin; NGAL; Lipocalin-2; SV-40-induced 24P3 protein; Siderocalin LCN2; p25; Lcn2

• Gene Name:

  Neutrophil gelatinase-associated lipocalin

• UniProt:

  P11672

• Host:

  Rabbit

• Reactivity:

  Mouse

• Cross Reactivity:

  No cross-reactivity with other proteins

• Immunogen:

  E. coli-derived mouse Lipocalin 2 recombinant protein (Position: Q21-N200) .

• Clonality:

  Polyclonal

• Tissue Specificity:

  Detected in lung, spleen, uterus, vagina and epididymis. .

• Applications:

  ELISA

• Field of Research:

  Immunoglobulins, Immunology

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

• Function:

  Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5- DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. .

• Storage Conditions:

  Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

• Fragment:

  Rabbit IgG

• Applications Notes:

  ELISA Capture (Matched Antibody Pair), 2-8μg/ml

• Other Gene Names:

  Lcn2

• Subcellular Location:

  Secreted . Upon binding to the SLC22A17 (24p3R) receptor, it is internalized.

• Protein Name:

  Neutrophil gelatinase-associated lipocalin