Anti-HMBS Antibody Picoband®

• Background:

  Porphobilinogen deaminase (hydroxymethylbilane synthase, or uroporphyrinogen I synthase) is an enzyme that in humans is encoded by the HMBS gene. This gene encodes a member of the hydroxymethylbilane synthase superfamily. The encoded protein is the third enzyme of the heme biosynthetic pathway and catalyzes the head to tail condensation of four porphobilinogen molecules into the linear hydroxymethylbilane. Mutations in this gene are associated with the autosomal dominant disease acute intermittent porphyria. Alternatively spliced transcript variants encoding different isoforms have been described.

• Description:

  Boster Bio Anti-HMBS Antibody Picoband® catalog # A01506-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Porphobilinogen deaminase; PBG-D; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase; HMBS; PBGD; UPS

• Gene Name:

  Hydroxymethylbilane synthase

• UniProt:

  P08397

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Cross Reactivity:

  No cross-reactivity with other proteins.

• Immunogen:

  E. coli-derived human HMBS recombinant protein (Position: N6-H361) .

• Clonality:

  Polyclonal

• Tissue Specificity:

  Isoform 1 is ubiquitously expressed. Isoform 2 is found only in erythroid cells.

• Applications:

  ELISA, WB

• Field of Research:

  Cell Biology, Cofactors, Vitamins/Minerals, Metabolism, Pathways and Processes, Signal Transduction, Vitamins/Minerals

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

• Function:

  Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.

• Storage Conditions:

  Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

• Fragment:

  Rabbit IgG

• Applications Notes:

  Western blot,0.1-0.5μg/ml
  ELISA,0.1-0.5μg/ml
  

• Other Gene Names:

  HMBS

• Subcellular Location:

  Cytoplasm.