Anti-MUC2 Antibody

• Background:

  Mucin 2, also known as MUC2, is a protein that in humans is encoded by the MUC2 gene. This gene encodes a member of the mucin protein family. It is mapped to 11p15.5. Mucin 2 is particularly prominent in the gut where it is secreted from goblet cells in the epithelial lining into the lumen of the large intestine. There, mucin 2, along with small amounts of related-mucin proteins, polymerizes into a gel of which 80% by weight is oligosaccharide side-chains that are added as post-translational modifications to the mucin proteins. This gel provides an insoluble mucous barrier that serves to protect the intestinal epithelium. The primary function of the MUC2 gene product is to provide a protective barrier between the epithelial surfaces and the gut lumen. There is decreased expression of MUC2 in colonic cancer and defective polymerization of secreted mucin in ulcerative colitis.

• Description:

  Anti-MUC2

• Synonyms:

  Mucin-2; MUC-2; Intestinal mucin-2; MUC2; SMUC

• Gene Name:

  Mucin 2, oligomeric mucus/gel-forming

• UniProt:

  Q02817

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Cross Reactivity:

  No cross-reactivity with other proteins.

• Immunogen:

  A synthetic peptide corresponding to a sequence at the N-terminus of human MUC2, which shares 86.1% amino acid (aa) sequence identity with both mouse and rat MUC2.

• Clonality:

  Polyclonal

• Tissue Specificity:

  Colon, small intestine, colonic tumors, bronchus, cervix and gall bladder.

• Applications:

  IF, IHC

• Field of Research:

  Cancer, Cell Type Markers, Cytoskeleton/ECM, ECM Proteins, Extracellular Matrix, Invasion/Microenvironment, Signal Transduction, Tags & Cell Markers, Tumor Associated, Tumor Immunology, Tumor-Associated Antigens

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

• Function:

  Coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer.

• Storage Conditions:

  Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

• Fragment:

  Rabbit IgG

• Applications Notes:

  Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat
  Immunofluorescence, 5μg/ml, Human, Mouse
  

• Other Gene Names:

  MUC2

• Subcellular Location:

  Secreted. In the intestine, secreted into the inner and outer mucus layers.

• Protein Name:

  Vinculin