Anti-Stefin B/CSTB Antibody Picoband®

• Background:

  Cystatin B (CSTB), also called STFB, is a small protein that is a member of the superfamily of cysteine protease inhibitors. It has been isolated from human spleen and liver and its amino acid sequence has been fully determined. The cystatin B gene is located on 21q22.3. It is widely distributed and is localized mostly intracellularly, but has been found extracellularly. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. Its role is thought to be as a protector against the proteinases leaking from lysosomes. A cystatin B multiprotein complex might have a specific cerebellar function, and that the loss of this function might contribute to the etiopathogenesis of EPM1. Upon differentiation to myotubes, CSTB becomes excluded from the nucleus and lysosomes, suggesting that the subcellular distribution of CSTB is dependent on the differentiation status of the cell.

• Description:

  Boster Bio Anti-Stefin B/CSTB Antibody Picoband® catalog # PB9565. Tested in ELISA, Flow Cytometry, IF, IHC ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Cystatin-B; CPI-B; Liver thiol proteinase inhibitor; Stefin-B; CSTB; CST6, STFB

• Gene Name:

  Cystatin-B

• UniProt:

  P04080

• Host:

  Rabbit

• Reactivity:

  Human

• Cross Reactivity:

  No cross-reactivity with other proteins

• Immunogen:

  E. coli-derived human Stefin B recombinant protein (Position: M1-F98) . Human Stefin B shares 78.6 % amino acid (aa) sequence identity with both mouse and rat Stefin B.

• Clonality:

  Polyclonal

• Tissue Specificity:

  Expressed in the skin throughout the epidermis. .

• Applications:

  ELISA, Flow Cytometry, IF, IHC, ICC, WB

• Field of Research:

  Cell Biology, Protease Inhibitors, Proteolysis/Ubiquitin

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

• Function:

  This is an intracellular thiol proteinase inhibitor. Tightly binding reversible inhibitor of cathepsins L, H and B.

• Storage Conditions:

  Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

• Fragment:

  Rabbit IgG

• Applications Notes:

  6

• Other Gene Names:

  CSTB

• Subcellular Location:

  Cytoplasm . Nucleus .

• Sequence Similarities:

  Belongs to the cystatin family.

• Protein Name:

  Cystatin-B