Biotinylated Human HLA-DRA1*01:01&HLA-DRB1*11:01 Monomer Protein (Peptide free, MALS verified)

Biotinylated Human HLA-DRA1*01:01&HLA-DRB1*11:01 Monomer Protein (Peptide free, MALS verified)

• Description: Biotinylated Human HLA-DRA1*01:01&HLA-DRB1*11:01 Monomer Protein (HL1-H82W6) is expressed from human 293 cells (HEK293). It contains AA Ile 26 - Glu 216 (HLA-DRA1*01:01) & Gly 30 - Lys 227 (HLA-DRB1*11:01) (Accession # CAI2388006.1 (HLA-DRA1*01:01) & CAI2388099.1 (HLA-DRB1*11:01)).
• Specifications: Biotinylated Human HLA-DRA1*01:01&HLA-DRB1*11:01 Monomer Protein, produced by co-expression of HLA-DRA1*01:01 and HLA-DRB1*11:01, has a calculated MW of 29.2 kDa (HLA-DRA1*01:01) and 29.2 kDa (HLA-DRB1*11:01). Subunit HLA-DRA1*01:01 is fused with a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) and subunit HLA-DRB1*11:01 contains no tag. The protein migrates as 40-43 kDa and 35-38 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression Region: Ile 26 - Glu 216 (HLA-DRA1*01:01) & Gly 30 - Lys 227 (HLA-DRB1*11:01)
• Host: HEK 293
• Target: HLA-DRA1*0101 & HLA-DRB1*1101
• Conjugation: Biotin-labeled
• Tag: C-10×His & C-Avi
• Source: Human
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Purity: 90%
• Bioactivity: Immobilized Biotinylated Human HLA-DRA1*01:01&HLA-DRB1*11:01 Monomer Protein (Cat. No. HL1-H82W6) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA-DR Antibody, Human IgG1 with a linear range of 0.06-2 ng/mL (QC tested).
• Format: Powder
• Buffer: PBS, pH7.4
• Additives: Trehalose
• Molecular Weight: 29.2 kDa and 29.2 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C