Biotinylated Human HLA-A*24:02&B2M&NY-ESO-1 (LLMWITQCF) Complex Protein (Monomer, MALS verified)

• Background:

  NY-ESO-1, which is also well-known as New York esophageal squamous cell carcinoma 1, is an efficient target for cancer immunotherapy. This antigen is a member of cancer-testis antigens (CTAs) and is highly expressed in various cancers, including melanoma, ovarian, cervical cancer, etc. Adoptive T cell therapy with HLA-A24 restricted NY-ESO-1 transduced CD8+ T cells has improved the clinical response rates and overall survival of treatment-refractory melanoma patients. The Human HLA-A*2402 NY-ESO-1 (LLMWITQCF) complex protein is a complex of HLA-A*2402 of the MHC Class I, B2M and LLMWITQCF peptide of the NY-ESO-1.

• Description:

  Biotinylated Human HLA-A*24:02&B2M&NY-ESO-1 (LLMWITQCF) Complex Protein (HL1-H82E3) is expressed from human 293 cells (HEK293) . It contains AA Gly 25 - Thr 305 (HLA-A*24:02) & Ile 21 - Met 119 (B2M) & LLMWITQCF peptide (Accession # AAA59600.1 (HLA-A*24:02) & P61769 (B2M) & LLMWITQCF) .

• Specifications:

  Biotinylated Human HLA-A*24:02&B2M&NY-ESO-1 (LLMWITQCF) Complex Protein is produced by co-expression of HLA and B2M loaded with NY-ESO-1 peptide. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 35.8 kDa and 11.7 kDa. The protein migrates as 38-42 kDa and <14 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

• Accession Number:

  AAA59600.1 (HLA-A*24:02) & P61769 (B2M) & LLMWITQCF

• Expression Region:

  Gly 25 - Thr 305 (HLA-A*24:02) & Ile 21 - Met 119 (B2M) & LLMWITQCF peptide

• Host:

  HEK293

• Target:

  HLA-A*2402 & B2M & NY-ESO-1 (LLMWITQCF)

• Conjugation:

  Biotin-labeled

• Tag:

  C-10xHis & C-Avi

• Source:

  Human

• Stability:

  ● The product MUST be stored at -70°C or lower upon receipt; ● -70°C for 3 months under sterile conditions.

• Endotoxin:

  1.0 EU per μg

• Purity:

  90%

• Bioactivity:

  Immobilized Biotinylated Human HLA-A*24:02&B2M&NY-ESO-1 (LLMWITQCF) Complex Protein (Cat. No. HL1-H82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.1-1 ng/mL (QC tested).

• Format:

  Liquid

• Buffer:

  PBS, pH7.4

• Additives:

  Trehalose

• Molecular Weight:

  35.8 kDa and 11.7 kDa

• Shipping Conditions:

  Dry ice

• Storage Conditions:

  -70°C

• Package Size:

  25ug*1

• Species:

  Human