Human Matriptase / ST14 Catalytic Domain Protein, Tag Free (active enzyme)

Human Matriptase / ST14 Catalytic Domain Protein, Tag Free (active enzyme)

• Background: Degrades extracellular matrix. Proposed to play a role in breast cancer invasion and metastasis. Exhibits trypsin-like activity as defined by cleavage of synthetic substrates with Arg or Lys as the P1 site. Involved in the terminal differentiation of keratinocytes through prostasin (PRSS8) activation and filaggrin (FLG) processing; Cleaves various synthetic substrates with Arg or Lys at the P1 position and prefers small side-chain amino acids, such as Ala and Gly, at the P2 position.
• Description: Human Matriptase / ST14 Catalytic Domain Protein, Tag Free (MAE-H5143) is expressed from E. coli cells. It contains AA Gly 596 - Val 855 (Accession # NP_068813).
• Specifications: This protein carries no "tag". The protein has a calculated MW of 30.6 kDa. The protein migrates as 27-28 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
• Expression Region: Gly 596 - Val 855
• Host: E. coli
• Target: Matriptase
• Conjugation: Unconjugated
• Tag: Native
• Source: Human
• Stability: The product MUST be stored at -70°C or lower upon receipt; -70°C for 3 months under sterile conditions.
• Endotoxin: 1.0 EU per μg
• Purity: 90%
• Format: Liquid
• Buffer: 50 mM Tris, 150 mM NaCl, 10 % Trehalose, pH7.5
• Additives: Glycerol
• Molecular Weight: 30.6 kDa
• Shipping Conditions: Dry ice
• Storage Conditions: -70°C