Biotinylated Human HLA-E*01:03&B2M&CMV UL40 (VMAPRTVLL) Complex Protein (Monomer, MALS verified)

Biotinylated Human HLA-E*01:03&B2M&CMV UL40 (VMAPRTVLL) Complex Protein (Monomer, MALS verified)

• Background: HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail.
• Description: Biotinylated Human HLA-E*01:03&B2M&CMV UL40 (VMAPRTVLL) Complex Protein (HL0-H82E7) is expressed from human 293 cells (HEK293). It contains AA Gly 22 - Ile 305 (HLA-E*01:03) & Ile 21 - Met 119 (B2M) & VMAPRTVLL peptide (Accession # P13747 (HLA-E*01:03) & P61769-1 (B2M) & VMAPRTVLL).
• Specifications: Biotinylated Human HLA-E*01:03&B2M&CMV UL40 (VMAPRTVLL) Complex Protein is produced by co-expression of HLA and B2M loaded with CMV UL40 peptide. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 36.2 kDa and 13.7 kDa. The protein migrates as 40-43 kDa and 12 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression Region: Gly 22 - Ile 305 (HLA-E*01:03) & Ile 21 - Met 119 (B2M) & VMAPRTVLL peptide
• Host: HEK 293
• Target: HLA-E*0103 & B2M & CMV UL40 (VMAPRTVLL)
• Conjugation: Biotin-labeled
• Tag: C-10×His & C-Avi
• Source: Human
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Purity: 95%
• Format: Powder
• Buffer: PBS, pH7.4
• Additives: Trehalose
• Molecular Weight: 36.2 kDa and 13.7 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C