ResDetect™ Universal Protein A Quick ELISA kit

• Background:

  Protein A is a cell wall protein of Staphylococcus aureus. Due to its high affinity with the Fc part of certain immunoglobulins (especially IgG), it is widely used in the purification of antibody drugs. However, during the purification , protein A may leach from the purification column and resulte in contamination of the antibody drugs prepared. Protein A has a variety of specific biological characteristics, so there are strict regulations on the acceptable level of Protein A in antibody drug preparations. Therefore, the detection of residual Protein A in antibody drugs purified from Protein A purification column is a key quality control step in the production process of antibody drug preparations.

• Description:

  ResDetect™ Universal Protein A Quick ELISA kit

• Target:

  Protein A (SuRe)

• Applications:

  The kit is developed for the detection of natural or structurally conserved recombinant forms of Protein A and alkaline-resistant Protein A variants, such as MabSelect SuRe™ , MaXtar® ARPA ligand (Bio-Link Co.), etc. in bioprocess manufacturing applications. It is used as a universal protein A and variants ligand detection tool to aid in optimal antibody purification process development and in routine quality control of in-process streams as well as final product.

• Stability:

  Unopened kit should be stored at 2°C-8°C upon receiving. Find the expiration date on the outside packaging and do not use reagents past their expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

• Format:

  Solid

• Components:

  The Protein A ELISA kit is used to measure the levels of protein A by employing a standard sandwich-ELISA format. The micro-plate in the kit has been pre-coated with a chicken anti-protein A polyclonal antibody. Firstly, the standard samples provided in kit and your samples are heated in a dry heating block or boiling water bath to denature and precipitate the product antibodies, after a centrifugation step to pellet the denatured product antibody, add the standard samples and your samples supernatant to the plate, then add the Biotin-Anti-Protein A Antibody to the plate and form Antibody-antigen (Protein A) - biotinylated antibody complex, incubate and wash the wells. Next add Horseradish peroxidase conjugated streptavidin (Streptavidin-HRP) to the plate, incubate and wash the wells to remove any unbound reactants. At last, load the tetramethylbenzidine (TMB) substrate into the wells and monitor a blue color. The reaction is stopped by the addition of a stop solution and the color turns yellow. The intensity of the absorbance can be measured at 450nm and 630nm on a microtiter plate reader. The OD Value reflects the amount of protein A.

• Shipping Conditions:

  Blue ice

• Storage Conditions:

  2-8°C

• Package Size:

  96tests*1

• CAS Number:

  7732-18-5