Anti-Phospho-IRF-3 (S385) Antibody

• Description:

  Boster Bio Anti-Phospho-IRF-3 (S385) Antibody catalog # A00165S385. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human.

• Synonyms:

  Interferon regulatory factor 3; IRF-3; IRF3;

• Gene Name:

  IRF3

• UniProt:

  Q14653

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  The antiserum was produced against synthesized peptide derived from human IRF-3 around the phosphorylation site of Ser385. AA range:351-400

• Clonality:

  Polyclonal

• Tissue Specificity:

  Expressed constitutively in a variety of tissues.

• Applications:

  WB, IHC, IF, ELISA

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration:

  1 mg/ml

• Form:

  Liquid

• Function:

  Key transcriptional regulator of type I interferon (IFN) -dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.

• Molecular Weight:

  47219 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

• Fragment:

  IgG

• Other Gene Names:

  Interferon regulatory factor 3

• Subcellular Location:

  Cytoplasm

• Protein Name:

  Interferon regulatory factor 3