Anti-EZH2 Antibody

• Background:

  EZH2 was initially identified as a homolog of the drosophila Enhancer of Zeste through exon trap screening of chromosome 21. Both EZH2 and the related protein EZH1 can form complexes with the noncanonical Polycomb repressive complex-2 (PRC2) and maintain repressive chromatin, but the PRC2-EZH1 complex mediates methylation of histone H3. Both EZH1 and EZH2 are thought to function in the maintenance of embryonic stem cell pluripotency and plasticity and recently have been shown to be essential for hair follicle homeostasis and wound repair. Overexpression of EZH2 has been reported as a marker for advanced and metastatic cancers.

• Description:

  Boster Bio Anti-EZH2 Antibody (Catalog # A00050) . Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse.

• Synonyms:

  WVS, ENX1, EZH1, KMT6, WVS2, ENX-1, EZH2b, KMT6A, Histone-lysine N-methyltransferase EZH2

• Gene Name:

  EZH2

• UniProt:

  Q15910

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Cross Reactivity:

  Multiple isoforms of EZH2 are known to exist. EZH2 antibody is predicted to not cross-react with EZH1.

• Immunogen:

  EZH2 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human EZH2. The immunogen is located within amino acids 190 - 240 of EZH2.

• Clonality:

  Polyclonal

• Tissue Specificity:

  Expressed in many tissues. Overexpressed in numerous tumor types including carcinomas of the breast, colon, larynx, lymphoma and testis.

• Applications:

  ELISA, WB, ICC, IF

• Field of Research:

  Stem Cells

• Purification:

  EZH2 Antibody is affinity chromatography purified via peptide column.

• Concentration:

  1 mg/mL

• Form:

  Liquid

• Function:

  Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys- 27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Displays a preference for substrates with less methylation, loses activity when progressively more methyl groups are incorporated into H3K27, H3K27me0 > H3K27me1 > H3K27me2 (PubMed:22323599) . Compared to EZH1-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non- histone proteins such as the transcription factor GATA4 and the nuclear receptor RORA. Regulates the circadian clock via histone methylation at the promoter of the circadian genes. Essential for the CRY1/2-mediated repression of the transcriptional activation of PER1/2 by the CLOCK-ARNTL/BMAL1 heterodimer; involved in the di and trimethylation of 'Lys-27' of histone H3 on PER1/2 promoters which is necessary for the CRY1/2 proteins to inhibit transcription.

• Molecular Weight:

  85363 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  EZH2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Other Gene Names:

  Enhancer of zeste homolog 2

• Subcellular Location:

  Nucleus

• Protein Name:

  Enhancer of zeste homolog 2