Anti-Interleukin-27 subunit beta EBI3 Antibody

• Background:

  EBI3 is a subunit in two distinct heterodimeric cytokines: interleukin-27 (IL-27) and IL-35. Like interleukin-23 (IL-23), IL-27 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. It exists as a heterodimer composed of the p40-related protein EBI3 and an IL-12 p35-related protein termed p28. IL-27 is produced after activation by antigen-presenting cells and induces proliferation of naïve but not memory CD4+ T-cells. It acts by binding to its receptor WSX-1 (also known as TCCR) and gp130 which results in the activation of a Jak/STAT signaling cascade, suggesting the IL-27 is involved in the regulation of immune processes. It has been suggested that IL-27 can also be used as a therapeutic agent against cancer as it can also induce tumor-specific anti-tumor activity mediated through CD8+ T-cells, IFN-gamma, and T-bet. IL-35 is composed of EBI3 and the p35 subunit of IL-12 and has been reported to have therapeutic effects against collagen-induced arthritis by expanding the population of regulatory T cells and suppressing Th17 cells. At least two isoform of EBI3 are known to exist.

• Description:

  Boster Bio Anti-Interleukin-27 subunit beta EBI3 Antibody (Catalog # A07190) . Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.

• Synonyms:

  IL27B, IL-27B, IL27B, Interleukin-27 subunit beta, Epstein-Barr virus-induced gene 3 protein, IL-27 subunit beta

• Gene Name:

  EBI3

• UniProt:

  Q14213

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Anti-EBI-3 antibody was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of mature human EBI-3. The immunogen is located within the last 50 amino acids of EBI3.

• Clonality:

  Polyclonal

• Tissue Specificity:

  Ubiquitous. Highly expressed in liver, kidney and muscle. Expressed at intermediate level in brain, heart, colon, thymus, kidney, small intestine, placenta, lung, leukocytes and spleen.

• Applications:

  ELISA, WB, IHC-P, IF

• Field of Research:

  Immunology

• Purification:

  EBI3 Antibody is affinity chromatography purified via peptide column.

• Concentration:

  1 mg/mL

• Form:

  Liquid

• Function:

  Associates with IL27 to form the IL-27 interleukin, a heterodimeric cytokine which functions in innate immunity. IL-27 has pro- and anti-inflammatory properties, that can regulate T- helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17) . It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines.

• Molecular Weight:

  25396 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  EBI3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Other Gene Names:

  Epstein-Barr virus induced 3

• Subcellular Location:

  Secreted

• Prediction Reactivity:

  Mouse, Rat

• Protein Name:

  Epstein-Barr virus induced 3