Anti-RICK RIPK2 Antibody

• Background:

  Apoptosis is mediated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. DD-containing serine/threonine kinase RIP regulates Fas-induced apoptosis. A novel CARD-containing serine/threonine kinase was recently identified and designated RICK/RIP2/CARDIAK for RIP-like interacting CLARP kinase, receptor interacting protein-2, and CARD-containing ICE associated kinase, respectively. RICK contains an N-terminal kinase catalytic domain and a C-terminal CARD domain. Overexpression of RICK induced apoptosis and activation of NF-κB and JNK. RICK interacts with members of the TRAF family, CLARP and caspase-1. Thus, RICK represents a novel kinase that regulates TNF and Fas induced-apoptosis and that is involved in the generation of proinflammatory cytokine IL-1β . The messenger RNA of RICK is expressed in multiple human tissues.

• Description:

  Boster Bio Anti-RICK RIPK2 Antibody (Catalog # A00818) . Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.

• Synonyms:

  CCK, RICK, RIP2, CARD3, GIG30, CARDIAK, UNQ277/PRO314/PRO34092, CARD-containing interleukin-1 beta-converting enzyme-associated kinase, CARD-containing IL-1 beta ICE-kinase

• Gene Name:

  RIPK2

• UniProt:

  O43353

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  RICK antibody was raised against a peptide corresponding to 20 amino acids near the amino terminus of human RICK. The immunogen is located within the first 50 amino acids of RICK.

• Clonality:

  Polyclonal

• Tissue Specificity:

  Detected in heart, brain, placenta, lung, peripheral blood leukocytes, spleen, kidney, testis, prostate, pancreas and lymph node.

• Applications:

  ELISA, WB, ICC, IF

• Field of Research:

  Apoptosis

• Purification:

  RICK Antibody is affinity chromatography purified via peptide column.

• Concentration:

  1 mg/ml

• Form:

  Liquid

• Function:

  Serine/threonine/tyrosine kinase that plays an essential role in modulation of innate and adaptive immune responses. Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize and recruit RIPK2 through CARD-CARD domains. Contributes to the tyrosine phosphorylation of the guanine exchange factor ARHGEF2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Once recruited, RIPK2 autophosphorylates and undergoes 'Lys-63'-linked polyubiquitination by E3 ubiquitin ligases XIAP, BIRC2 and BIRC3. The polyubiquitinated protein mediates the recruitment of MAP3K7/TAK1 to IKBKG/NEMO and induces 'Lys-63'-linked polyubiquitination of IKBKG/NEMO and subsequent activation of IKBKB/IKKB. In turn, NF-kappa-B is released from NF-kappa-B inhibitors and translocates into the nucleus where it activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Plays also a role during engagement of the T-cell receptor (TCR) in promoting BCL10 phosphorylation and subsequent NF-kappa-B activation.

• Molecular Weight:

  61195 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  RICK antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Other Gene Names:

  Receptor-interacting serine-threonine kinase 2

• Subcellular Location:

  Cytoplasm .

• Prediction Reactivity:

  Bovine

• Protein Name:

  Receptor-interacting serine-threonine kinase 2